96594-10-4

Basic information

• Product Name: H-ALA-AMC TFA
• Synonyms: L-Alanine-4-methyl-7-coumarinylamide trifluoroacetate salt ;L-Alanine 7-amido-4-methylcoumarin trifluoroacetat;L-ALANINE-7-AMINO-4-METHYLCOUMARIN TRIFLUOROACETATE SALT;L-Alanine-4-methyl-7-coumarinylamide trifluoroacetateL-Alanine-7-amido-4-methylcoumarin trifluoroacetate salt;L-Alanine-7-amido-4-methylcoumarin trifluoroacetic acid salt;(S)-2-Amino-N-(4-methyl-2-oxo-2H-chromen-7-yl)propanamide 2,2,2-trifluoroacetate;L-Ala-AMC·TFA;L-Alanine 7-amido-4-methylcoumarin trifluoroacetate≥ 99% (HPLC)
• CAS NO: 96594-10-4
• Molecular Formula: C15H15F3N2O5
• Molecular Weight: 360.29
• Product Categories: substrate
• Mol File: 96594-10-4.mol
• Article Data: 0

Chemical Properties

• Melting Point: 200-206 °C
• Boiling Point: 490.1 °C at 760 mmHg
• Flash Point: 250.2 °C
• Appearance: /
• Vapor Pressure: 9.41E-10mmHg at 25°C
• Storage Temp.: −20°C
• Solubility: ethanol: 50 mg/mL, clear, colorless
• CAS DataBase Reference: H-ALA-AMC TFA(CAS DataBase Reference)
• NIST Chemistry Reference: H-ALA-AMC TFA(96594-10-4)
• EPA Substance Registry System: H-ALA-AMC TFA(96594-10-4)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 96594-10-4(Hazardous Substances Data)

Usage

Description

H-ALA-AMC TFA is a fluorogenic peptidase substrate that is used to study the activity of peptidases. It has a hydrolysis reaction that results in a longer wavelength excitation and emission spectrum, with an excitation wavelength of 380 nm and an emission wavelength of 440 nm.

Uses

Used in Research Applications:
H-ALA-AMC TFA is used as a research tool for studying the activity of peptidases. Its fluorogenic properties allow for the detection and quantification of peptidase activity, making it a valuable tool for researchers in the field of enzymology and proteomics.
Used in Drug Discovery and Development:
H-ALA-AMC TFA is used as a substrate in assays for the identification and characterization of potential drug candidates that target peptidases. Its ability to produce a fluorescent signal upon hydrolysis allows for the screening of compounds that can modulate peptidase activity, aiding in the development of new therapeutic agents.
Used in Diagnostic Applications:
H-ALA-AMC TFA can be used as a diagnostic tool to assess peptidase activity in biological samples. Abnormal peptidase activity can be indicative of certain diseases or conditions, and the use of H-ALA-AMC TFA can help in the diagnosis and monitoring of these conditions.
Used in Quality Control and Validation:
H-ALA-AMC TFA is used in quality control and validation processes to ensure the accuracy and reliability of peptidase assays. Its consistent fluorescence properties make it an ideal substrate for assessing the performance of assay systems and validating new methods for peptidase analysis.

InChI:InChI=1/C13H14N2O3.C2HF3O2/c1-7-5-12(16)18-11-6-9(3-4-10(7)11)15-13(17)8(2)14;3-2(4,5)1(6)7/h3-6,8H,14H2,1-2H3,(H,15,17);(H,6,7)/t8-;/m0./s1