99792-79-7

Basic information

• Product Name: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID
• Synonyms: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID;CHLOROPHENOL RED BETA-D-GALACTOPYRANOSIDE (CPRG);Chlorophenolred-b-D-galactopyranoside;chlorophenol red galactopyranoside;Chlorophenol Red-β-D-galactopyranoside ,90%;beta-D-Galactopyranoside 2-chloro-4-[3-(3-chloro-4-hydroxyphenyl)-1,1-dioxido-3H-2,1-benzoxathiol-3-yl]phenyl;Chlorophenol Red-β-D-galactoside
• CAS NO: 99792-79-7
• Molecular Formula: C₂₅H₂₂Cl₂O₁₀S
• Molecular Weight: 585.40718
• EINECS: 1592732-453-0
• Mol File: 99792-79-7.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 791.4 °C at 760 mmHg
• Flash Point: 432.4 °C
• Appearance: /
• Density: 1.661
• Vapor Pressure: 2.13E-26mmHg at 25°C
• Refractive Index: 1.697
• Storage Temp.: −20°C
• CAS DataBase Reference: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID(CAS DataBase Reference)
• NIST Chemistry Reference: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID(99792-79-7)
• EPA Substance Registry System: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID(99792-79-7)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 99792-79-7(Hazardous Substances Data)

Usage

Description

Chlorophenol red β-D-galactopyranoside (CPRG) is a colorimetric substrate specifically designed for the detection and quantification of β-galactosidase activity. CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID serves as an indicator of enzymatic activity, allowing for the measurement of β-galactosidase expression levels in various applications.

Uses

Used in Molecular Biology and Biotechnology:
Chlorophenol Red-β-D-galactopyranoside is used as a substrate for β-galactosidase to measure the expression levels of the enzyme in transfected cells. This application is crucial for studying gene expression and the functionality of recombinant proteins in various biological systems.
Used in Diagnostics and Clinical Research:
In the field of diagnostics and clinical research, Chlorophenol Red-β-D-galactopyranoside serves as a colorimetric substrate for the detection of multiple clinically relevant mutations using a lacZ output gene. This allows for the identification and analysis of genetic variations that may have implications for disease progression or treatment response.
Used in Microbiology:
Chlorophenol Red-β-D-galactopyranoside is utilized as a substrate for β-galactosidase in colorimetric assays for bacteria quantification. This application aids in the assessment of bacterial growth and the study of bacterial metabolism, which is essential for understanding microbial dynamics in various environments.
Used in Paper-based Cell-free Protein Expression Systems:
Chlorophenol Red-β-D-galactopyranoside has been employed as a substrate in paper-based cell-free protein expression systems for target RNA detection. This innovative approach allows for the detection of specific RNA sequences, which can be valuable in molecular diagnostics and the study of gene regulation.
Used in Colorimetric Determination and ELISA:
Chlorophenol Red-β-D-galactopyranoside is used in colorimetric determination of β-Gal activity in cell extracts and enzyme-linked immunosorbent assays (ELISA). These techniques are widely used in research and clinical settings to quantify enzyme activity and analyze protein-protein interactions, contributing to a better understanding of biological processes and disease mechanisms.

Biochem/physiol Actions

Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.

InChI:InChI=1/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)25(14-3-1-2-4-20(14)38(33,34)37-25)13-6-8-18(16(27)10-13)35-24-23(32)22(31)21(30)19(11-28)36-24/h1-10,19,21-24,28-32H,11H2/t19-,21+,22+,23-,24-,25?/m1/s1

Synthetic route

chlorophenol red mono-β-D-galactopyranoside (99792-79-7) → chlorophenol red

Conditions	Yield
With β-galactosidase at 25℃; pH=7.4; aq. phosphate buffer;