Add time:08/29/2019 Source:sciencedirect.com
The LACTO-N-NEOTETRAOSE (cas 13007-32-4) tetrasaccharide was synthesized on a new dendrimeric support, based on polyethylene glycol. Starting from 1-thio-β-d-lactose, the trisaccharide (2-acetamido-2-deoxy-β-d-glucopyranosyl)-(1 → 3)-O-β-d-galactopyranosyl-(1 → 4)-1-thio-β-d-glucopyranose was obtained using Neisseria meningitidis β-(1 → 3)-N-acetylglucosaminyltransferase according to a soluble synthesis approach, bound on the support and galactosylated using the milk β-(1 → 4)-galactosyl transferase to give after cleavage the tetrasaccharide lacto-N-neotetraose.
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