102029-52-7 Usage
Purification Methods
Purification can be carried out by passage through a DEAE-cellulose formate column, then through a Dowex 50 (H+) column to remove Na ions, concentrated by lyophilisation and redissolved in H2O. It is commercially available as a solution of 0.05g/mL of H2O. The concentration of acetoacetylcoenzyme A is determined by the method of Stern et al. [J Biol Chem 221 15 1956]. It is stable at pH 7-7.5 for several hours at 0o (half-life ca 1-2hours). At room temperature it is hydrolysed in ca 1-2hours at pH 7-7.5. At pH 1.0/20o it is more stable than at neutrality. A solution of the trisodium salt (0.05g/mL H2O) adjusted to pH 5 with 2N NaOH can be stored frozen for several weeks. It is stable at pH 2-3/-17o for at least 6 months. [Hersch & Jencks J Biol Chem 242 3468 1967, Clikenbeard et al. J Biol Chem 250 3108 1975, Simon & Shemin J Am Chem Soc 75 2520 1953, Moffatt & Khorana 81 1265 1959, Salem et al. Biochem J 258 563 1989, Beilstein 26 III/IV 3668.]
Check Digit Verification of cas no
The CAS Registry Mumber 102029-52-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,2,0,2 and 9 respectively; the second part has 2 digits, 5 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 102029-52:
(8*1)+(7*0)+(6*2)+(5*0)+(4*2)+(3*9)+(2*5)+(1*2)=67
67 % 10 = 7
So 102029-52-7 is a valid CAS Registry Number.
InChI:InChI=1/C25H40N7O18P3S.3Na/c1-13(33)8-16(35)54-7-6-27-15(34)4-5-28-23(38)20(37)25(2,3)10-47-53(44,45)50-52(42,43)46-9-14-19(49-51(39,40)41)18(36)24(48-14)32-12-31-17-21(26)29-11-30-22(17)32;;;/h11-12,14,18-20,24,36-37H,4-10H2,1-3H3,(H,27,34)(H,28,38)(H,42,43)(H,44,45)(H2,26,29,30)(H2,39,40,41);;;/q;3*+1/p-3/t14-,18-,19-,20?,24-;;;/m1.../s1
