102386-32-3Relevant articles and documents
Studies on Transfer Ribonucleic Acids (tRNA) and Related Compounds. XLIX. Solid-Phase Synthesis of Nonadecaribonucleotide Corresponding to the 5'-Half of the Formylmethionine tRNA Deficient in D-Loop and Stem Region
Ohtsuka, Eiko,Matsugi, Jitsuhiro,Yamane, Akio,Morioka, Hiroshi,Ikehara, Morio
, p. 4152 - 4159 (2007/10/02)
Ribooligonucleotides (nonamer, decamer and nonadecamer) were synthesized by the solid phase phosphotriester method using 2'-O-tetrahydrofuranyl nucleosides.These oligomers were designed in order to obtain E. coli tRNAfMet deficient in D-loop and D-stem and its analogs.Condensation was performed by using dimer blocks and the dimethoxytrityl group on the 5'-hydroxyl group was removed selectively by 1 M ZnBr2 without affecting the 2'-protection.Keywords - solid-phase synthesis; ribooligonucleotide; D-loop deficient tRNA; high pressure liquid chromatography; mobility shift analysis