102783-51-7 Usage
Description
2'-Deoxycytidine-5'-triphosphoric acid disodium salt, also known as dCTP, is a nucleoside triphosphate that serves as a crucial metabolite in the synthesis of DNA. It is a white powder with a clear colorless solution at a concentration of 100 mM and a pH of 8.3. dCTP is specially manufactured and purified to achieve the highest possible chemical purity, containing at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
Uses
Used in Molecular Biology and DNA Synthesis:
2'-Deoxycytidine-5'-triphosphoric acid disodium salt is used as a substrate for DNA synthesis with DNA polymerases and reverse transcriptases. It plays a vital role in various molecular biology methods, including polymerase chain reaction (PCR), DNA sequencing, and other DNA polymerase-driven reactions.
Used in PCR Applications:
In the polymerase chain reaction industry, 2'-Deoxycytidine-5'-triphosphoric acid disodium salt is used as a key component for amplifying specific DNA sequences. It is essential for the PCR process, as it provides the necessary energy and building blocks for the synthesis of new DNA strands.
Used in DNA Sequencing:
In the field of DNA sequencing, 2'-Deoxycytidine-5'-triphosphoric acid disodium salt is utilized as a nucleotide precursor for the incorporation of dCTP into the growing DNA strand. This allows for the determination of the order of nucleotides in a DNA molecule, which is crucial for understanding genetic information and identifying genetic variations.
Used in Research and Diagnostics:
2'-Deoxycytidine-5'-triphosphoric acid disodium salt is also used in research and diagnostic applications, where it aids in the study of DNA replication, repair, and other cellular processes. It is an essential tool for scientists and researchers working in the fields of genetics, molecular biology, and biotechnology.
Biochem/physiol Actions
2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase. It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.
Check Digit Verification of cas no
The CAS Registry Mumber 102783-51-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,2,7,8 and 3 respectively; the second part has 2 digits, 5 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 102783-51:
(8*1)+(7*0)+(6*2)+(5*7)+(4*8)+(3*3)+(2*5)+(1*1)=107
107 % 10 = 7
So 102783-51-7 is a valid CAS Registry Number.