1056640-28-8Relevant articles and documents
Light uncages a copper complex to induce nonapoptotic cell death
Kumbhar, Anupa A.,Franks, Andrew T.,Butcher, Raymond J.,Franz, Katherine J.
supporting information, p. 2460 - 2462 (2013/04/23)
Cu3G is a Cu(ii) complex of a photoactive tetradentate ligand that is cleaved upon UV irradiation to release Cu. Here we show that the cytotoxicity of Cu3G increases in response to brief UV stimulation to result in extensive cytoplasmic vacuolization that is indicative of nonapoptotic cell death.
A photolabile ligand for light-activated release of caged copper
Ciesienski, Katie L.,Haas, Kathryn L.,Dickens, Marina G.,Tesema, Yohannes T.,Franz, Katherine J.
supporting information; experimental part, p. 12246 - 12247 (2009/02/05)
A photosensitive caged copper complex has been prepared from a tetradentate ligand (H2cage) composed of two pyridyl-amide arms connected by a photoreactive nitrophenyl group. H2cage binds Cu2+ in aqueous solution with a stability constant (log β) of 10.8, which corresponds to a KD of 16 pM at pH 7.4. The neutral Cu2+ complex, [Cu(OH2)(cage)], crystallizes as a distorted trigonal bipyramid coordinated by two amide and two pyridyl N atoms, with a water molecule bound in the trigonal plane. Photolysis with 350 nm UV light cleaves the ligand backbone to release photoproducts with significantly diminished affinity for Cu2+, thereby uncaging the metal ion. When coordinated as the caged complex, copper has diminished reactivity to produce hydroxyl radicals from Fenton-like reaction mixtures containing hydrogen peroxide and ascorbic acid. Postphotolysis, uncaged copper promotes hydroxyl radical formation under the same conditions. The strategy of caging copper is promising for applications where light could be used to trigger release of copper as a pro-oxidant to increase oxidative stress or as a tool to release copper intracellularly to study mechanisms of copper trafficking. Copyright
