1089167-64-5

Basic information

• Product Name: 2-(2-(2-hydroxy-3,5-dinitrobenzoyl)hydrazono)acetic acid
• Synonyms: 2-(2-(2-hydroxy-3,5-dinitrobenzoyl)hydrazono)acetic acid
• CAS NO: 1089167-64-5
• Molecular Weight: 298.169
• Mol File: 1089167-64-5.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: 2-(2-(2-hydroxy-3,5-dinitrobenzoyl)hydrazono)acetic acid(CAS DataBase Reference)
• NIST Chemistry Reference: 2-(2-(2-hydroxy-3,5-dinitrobenzoyl)hydrazono)acetic acid(1089167-64-5)
• EPA Substance Registry System: 2-(2-(2-hydroxy-3,5-dinitrobenzoyl)hydrazono)acetic acid(1089167-64-5)

Safety Data

• Hazardous Substances Data: 1089167-64-5(Hazardous Substances Data)

Upstream product

• 955-07-7 2-Hydroxy-3,5-dinitrobenzoic acid hydrazide 
• 298-12-4 Glyoxilic acid 

Relevant articles and documents

Design and synthesis of immunoconjugates and development of an indirect ELISA for rapid detection of 3, 5-dinitrosalicyclic acid hydrazide

In this study novel immunoconjugates were designed, synthesized and then used to develop a rapid, specific and sensitive indirect ELISA method to directly detect residues of 3,5-dinitrosalicyclic acid hydrazide (DNSH), a toxic metabolite of nifursol present in chicken tissues. The hapten DNSHA was first designed and used to covalently couple to BSA to form an immunogen which was immunized to rabbits to produce a polyclonal antibody against DNSH. Furthermore, a novel 3,5-dinitrosalicylic acid-ovalbumin (DNSA-OVA) immunoconjugate structurally different from DNSHA-OVA was designed and used as a "substructural coating antigen" to improve the sensitivity of an indirect ELISA analysis for a direct DNSH detection. Based on the "substructural coating antigen" concept, an optimized indirect ELISA method was established that exhibited good specificity and high sensitivity for detecting DNSH, with a cross-reactivity of less than 0.1% (excluding the parent compound nifursol), IC50 of 0.217 nmol/mL and detection limit of 0.018 nmol/mL. Finally, a simple and efficient analysis of DNSH samples in chicken tissues showed that the average recovery rate of the indirect ELISA analysis was 82.3%, with the average coefficient of variation 15.9%. Thus, the developed indirect ELISA method exhibited the potential for a rapid detection of DNSH residues in tissue.