109276-34-8 Usage
Description
BIOTINAMIDOCAPROYL HYDRAZIDE is a hydrazide-activated biotinylation reagent specifically designed for labeling glycoproteins and carbohydrate-containing compounds with oxidizable sugars or aldehydes. It features a hydrazine moiety that reacts with an aldehyde to form semi-permanent hydrazone bonds, and an extended spacer arm that helps minimize steric hindrance. This reagent is characterized by a melting point of 195°C.
Uses
Used in Biochemical Research:
BIOTINAMIDOCAPROYL HYDRAZIDE is used as a biotinylation reagent for attaching biotin molecules to glycoproteins through the carbohydrate component. This is achieved by forming hydrazone bonds with aldehyde groups present in the oxidized sugars of the glycoproteins.
Used in Diagnostics and Detection:
In the field of diagnostics, BIOTINAMIDOCAPROYL HYDRAZIDE is used as a biotinylation reagent to label glycoproteins, enabling their detection and quantification in various assays. The biotinylated glycoproteins can be detected using streptavidin-conjugated probes, which have a high affinity for biotin.
Used in Drug Development:
BIOTINAMIDOCAPROYL HYDRAZIDE is employed in the development of drugs targeting glycoproteins, particularly in the identification and characterization of drug candidates that interact with specific glycoproteins. The biotinylated glycoproteins can be used in screening assays to identify potential drug candidates with high specificity.
Used in Analytical Techniques:
In analytical chemistry, BIOTINAMIDOCAPROYL HYDRAZIDE is used as a biotinylation reagent to label glycoproteins for various analytical techniques, such as mass spectrometry and chromatography. The biotinylated glycoproteins can be selectively captured and analyzed, providing valuable information about their structure and function.
Used in Pharmaceutical Industry:
BIOTINAMIDOCAPROYL HYDRAZIDE is used as a biotinylation reagent in the pharmaceutical industry for the development of targeted drug delivery systems. By biotinylating specific glycoproteins, these molecules can be selectively delivered to desired cellular or tissue targets, improving the efficacy and reducing the side effects of the drug.
Purification Methods
Suspend the hydrazide in ice-water (100mg/mL), stand overnight at 4o, filter and dry the solid in a vacuum. Recrystallise it from isoPrOH. RF is 0.26 on SiO2 plate using CHCl3/MeOH (7:3) as eluent. [O'Shannessy et al. Anal Biochem 163 204 1987.]
Check Digit Verification of cas no
The CAS Registry Mumber 109276-34-8 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,9,2,7 and 6 respectively; the second part has 2 digits, 3 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 109276-34:
(8*1)+(7*0)+(6*9)+(5*2)+(4*7)+(3*6)+(2*3)+(1*4)=128
128 % 10 = 8
So 109276-34-8 is a valid CAS Registry Number.
InChI:InChI=1/C16H29N5O3S/c17-21-14(23)8-2-1-5-9-18-13(22)7-4-3-6-12-15-11(10-25-12)19-16(24)20-15/h11-12,15H,1-10,17H2,(H,18,22)(H,21,23)(H2,19,20,24)/t11-,12-,15-/m0/s1
109276-34-8Relevant articles and documents
Design and synthesis of biotinylated cardiac glycosides for probing Nur77 protein inducting pathway
Tian, Dan-mei,Qiao, Jia,Bao, Yu-zhou,Liu, Jie,Zhang, Xiao-kun,Sun, Xue-long,Zhang, You-wei,Yao, Xin-sheng,Tang, Jin-shan
, p. 707 - 712 (2019/01/22)
The orphan nuclear receptor Nur77 (also known as TR3 or nerve growth factor-induced clone B NGFI-B) functions as a nuclear transcription factor in the regulation of target gene expression and plays a critical role in the regulation of differentiation, proliferation, apoptosis, and survival of many different cell types. Recent studies demonstrate that Nur77 also involves many important physiological and pathological processes including cancer, inflammation and immunity, cardiovascular diseases, and bone diseases. Our previous studies showed that cardiac glycosides could induce the expression of Nur77 protein and its translocation from the nucleus to the cytoplasm and subsequent targeting to mitochondria, leading to apoptosis of cancer cells. In order to probe the Nur77 protein inducting pathway, we designed and synthesized a series of novel biotinylated cardiac glycosides from β-Antiarin and α-Antiarin, two typical cardiac glycosides from the plant of Antiaris toxicaria. The induction of Nur77 protein expression of these biotinylated cardiac glycosides and their inhibitory effects on NIH-H460 cancer cell proliferation were evaluated. Results displayed that some biotinylated cardiac glycosides could significantly induce the expression of Nur77 protein comparable with their parent compounds β-Antiarin and α-Antiarin. Also, their streptavidin binding activities were evaluated. Among them, biotinylated cardiac glycosides P4b and P5a exhibited significant effect on the induction of Nur77 expression along with high binding capacity with streptavidin, suggesting that they can be used as probes for probing Nur77 protein inducting pathway.