112926-02-0 Usage
Description
INDO 1-AM is a cell-permeable derivative of INDO 1, which is a fluorescent probe used for detecting calcium ions (Ca2+). It is a yellow solid that exhibits a large shift in fluorescent emission from 482 nm to 398 nm upon binding with Ca2+. INDO 1-AM has a lower affinity for Ca2+ compared to FURA-2 but is still a valuable tool for various applications due to its cell permeability and rapid hydrolysis by cytoplasmic esterases into the membrane-impermeant INDO 1.
Uses
Used in Biomedical Research:
INDO 1-AM is used as a cell-permeable fluorescent probe for detecting and measuring intracellular calcium ions (Ca2+) in biomedical research. The application reason is its ability to cross cell membranes and be rapidly hydrolyzed by cytoplasmic esterases into the membrane-impermeant INDO 1, allowing for ratiometric fluorescence measurements.
Used in Neuroscience:
In neuroscience, INDO 1-AM is used as a fluorescent probe for studying calcium signaling in neurons and other excitable cells. The application reason is its sensitivity to changes in intracellular calcium levels, which play a crucial role in various neuronal processes, including neurotransmitter release, synaptic plasticity, and neuronal development.
Used in Cell Biology:
In cell biology, INDO 1-AM is used as a tool for investigating calcium signaling pathways and their role in cell function and dysfunction. The application reason is its ability to provide real-time measurements of intracellular calcium levels, which are essential for various cellular processes such as cell proliferation, differentiation, and apoptosis.
Used in Drug Discovery:
INDO 1-AM is used as a screening tool in drug discovery for identifying compounds that modulate calcium signaling pathways. The application reason is its ability to detect changes in intracellular calcium levels, which can be indicative of the effects of potential drug candidates on cellular processes involving calcium ions.
Check Digit Verification of cas no
The CAS Registry Mumber 112926-02-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,2,9,2 and 6 respectively; the second part has 2 digits, 0 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 112926-02:
(8*1)+(7*1)+(6*2)+(5*9)+(4*2)+(3*6)+(2*0)+(1*2)=100
100 % 10 = 0
So 112926-02-0 is a valid CAS Registry Number.
InChI:InChI=1/C33H34N2O17/c1-19(36)45-15-49-30(40)12-35(13-31(41)50-16-46-20(2)37)28-8-7-24(11-29(28)44-14-32(42)51-17-47-21(3)38)26-9-23-5-6-25(10-27(23)34-26)33(43)52-18-48-22(4)39/h5-11,34H,12-18H2,1-4H3
