117464-70-7 Usage
Description
BCECF-AM, also known as 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein, is a cell-permeable ester of BCECF that, upon hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. It is a widely-used fluorescent indicator for estimating intracellular pH and can be used to investigate intracellular changes in other ions, including potassium.
Used in Biomedical Research:
BCECF-AM is used as a fluorescent indicator for estimating intracellular pH in biomedical research. It allows simultaneous recording of cell volume changes and intracellular pH in single cells, making it a valuable tool for studying various cellular processes and mechanisms.
Used in Spectrofluorometry and Flow Cytometry:
BCECF-AM is used as a pH-sensitive dye in spectrofluorometry and flow cytometry for intracellular pH measurements. The pH-dependent ratio of emission intensity is determined when the dye is excited at different wavelengths (490 nm vs. 440 nm), providing a reliable method for assessing changes in intracellular pH.
Used in Drug Delivery Systems:
Although not explicitly mentioned in the provided materials, BCECF-AM could potentially be used in drug delivery systems to monitor the intracellular pH environment and optimize drug release or targeting.
Chemical Properties:
BCECF-AM is an isomeric mixture of lactones, acetates, and AM esters, and it appears as a pale yellow to yellow powder.
Biological Activity
Non-charged, non-fluorescent pH indicator. Readily and rapidly diffuses through cell membranes.
Check Digit Verification of cas no
The CAS Registry Mumber 117464-70-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,7,4,6 and 4 respectively; the second part has 2 digits, 7 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 117464-70:
(8*1)+(7*1)+(6*7)+(5*4)+(4*6)+(3*4)+(2*7)+(1*0)=127
127 % 10 = 7
So 117464-70-7 is a valid CAS Registry Number.
