118101-43-2Relevant articles and documents
Purification and Reaction of a New Enzyme, Nucleoside Oxidase
Isono, Yoshikazu,Sudo, Tomoko,Hoshino, Masami
, p. 1663 - 1670 (2007/10/02)
Nucleoside oxidase, a novel nucleoside oxidizing enzyme has been purified from a crude extract of Pseudomonas maltophilia LB-86 by a ammonium sulfate fractination, heat treatment, column chomatography on DEAE-Toyopearl, and gel filtration twice on Sephacryl S-200.The overall purification was approximately 60-fold with a yield of 35 percent.The purified enzyme gave a single protein band on acrylamide gel electrophoresis.Reaction products from inosine were identified as inosine-5'-aldehyde and inosine-5'-carboxylic acid.The enzyme catalyzed the oxidation of inosine to inosine-5'-carboxylic acid via inosine-5'-aldehyde using molecular oxygen as a primary electron acceptor with no formation of hydrogen peroxide.