118160-62-6Relevant articles and documents
Helical aggregates of bis(styryl) dyes formed by DNA templating
Fedorov, Yury V.,Fedorova, Olga A.,Shepel, Nikolai A.,Tokarev, Sergey D.,Tsvetkov, Vladimir B.,Ustimova, Maria A.
, (2021/06/09)
In order to further investigate mechanism of the styryl dye – DNA interaction, three different bis(styryl)pyridinium dyes possessing OMe or/and NMe2 substituents in phenyl ring were selected and interaction of these compounds with the calf thymus DNA (ct-DNA) was monitored by using of UV–vis and fluorescence spectroscopic techniques, circular dichroism (CD), Hoechst 33258 displacement experiments and quantum-chemical calculations. The experimental results indicated a higher fluorescence enhancement for dyes containing NMe2 group upon binding with DNA. The results proved the interaction of the molecules with ct-DNA occurs through the formation of aggregates in minor groove at high dye concentration. Bis(styryl) dye with OMe substituent forms the helical dye aggregates of right-handed chirality, whereas, dye containing NMe2 group demonstrates formation of left-handed chiral aggregates in minor groove of DNA. As the DNA concentration increases, the dyes begin to spread out along the minor groove. This research provided a better understanding of mechanism of styryl dye - DNA interaction and effect of dye structure on interacting mode with DNA.
