1225277-47-3Relevant articles and documents
Structure-Based Design of a Eukaryote-Selective Antiprotozoal Fluorinated Aminoglycoside
Kanazawa, Hiroki,Saavedra, Oscar M.,Maianti, Juan Pablo,Young, Simon A.,Izquierdo, Luis,Smith, Terry K.,Hanessian, Stephen,Kondo, Jiro
, p. 1541 - 1548 (2018)
Aminoglycosides (AG) are antibiotics that lower the accuracy of protein synthesis by targeting a highly conserved RNA helix of the ribosomal A-site. The discovery of AGs that selectively target the eukaryotic ribosome, but lack activity in prokaryotes, are promising as antiprotozoals for the treatment of neglected tropical diseases, and as therapies to read-through point-mutation genetic diseases. However, a single nucleobase change A1408G in the eukaryotic A-site leads to negligible affinity for most AGs. Herein we report the synthesis of 6′-fluorosisomicin, the first 6′-fluorinated aminoglycoside, which specifically interacts with the protozoal cytoplasmic rRNA A-site, but not the bacterial A-site, as evidenced by X-ray co-crystal structures. The respective dispositions of 6′-fluorosisomicin within the bacterial and protozoal A-sites reveal that the fluorine atom acts only as a hydrogen-bond acceptor to favorably interact with G1408 of the protozoal A-site. Unlike aminoglycosides containing a 6′-ammonium group, 6′-fluorosisomicin cannot participate in the hydrogen-bonding pattern that characterizes stable pseudo-base-pairs with A1408 of the bacterial A-sites. Based on these structural observations it may be possible to shift the biological activity of aminoglycosides to act preferentially as antiprotozoal agents. These findings expand the repertoire of small molecules targeting the eukaryotic ribosome and demonstrate the usefulness of fluorine as a design element.
Biomimetic synthesis and structural refinement of the macrocyclic dimer aminoglycoside 66-40C - The remarkably selective self-condensation of a putative aldehyde intermediate in the submerged culture medium producing sisomicin
Hanessian, Stephen,Maianti, Juan Pablo
supporting information; experimental part, p. 2013 - 2015 (2010/07/07)
Aminoglycoside 66-40C, an unprecedented 16-membered bis-azadiene macrocyclic natural product isolated from the Micromonospora producer of the antibiotic sisomicin, was synthesized following a biomimetic strategy which definitively established its origin as arising from a remarkably selective non-enzymatic macro-dimerization. The Royal Society of Chemistry.
