1227633-49-9

Basic information

• Product Name: SteMRegenin 1
• Synonyms: SteMRegenin 1;4-(2-(2-(benzo[b]thiophen-3-yl)-9-isopropyl-9H-purin-6-ylaMino)ethyl)phenol;SR1;SR-1;Phenol, 4-[2-[[2-benzo[b]thien-3-yl-9-(1-Methylethyl)-9H-purin-6-yl]aMino]ethyl]-;etMRegenin 1;Novartis SR1;SteMRegenin 1 (SR1)
• CAS NO: 1227633-49-9
• Molecular Formula: C₂₄H₂₃N₅OS
• Molecular Weight: 429.53732
• Product Categories: apis;Antineoplastic;Inhibitors
• Mol File: 1227633-49-9.mol
• Article Data: 2

Chemical Properties

• Boiling Point: 604.9±65.0 °C(Predicted)
• Appearance: /
• Density: 1.36±0.1 g/cm3(Predicted)
• Storage Temp.: +2C to +8C
• Solubility: Soluble in DMSO (up to 10 mg/ml).
• PKA: 10.03±0.15(Predicted)
• Stability: Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 3 months.
• CAS DataBase Reference: SteMRegenin 1(CAS DataBase Reference)
• NIST Chemistry Reference: SteMRegenin 1(1227633-49-9)
• EPA Substance Registry System: SteMRegenin 1(1227633-49-9)

Safety Data

• Hazard Codes: Xn
• Statements: 22
• Hazardous Substances Data: 1227633-49-9(Hazardous Substances Data)

Usage

Description

SteMRegenin 1, also known as SR1, is a cell-permeable purine compound that functions as a high affinity AhR (arylhydrocarbon receptor) antagonist. It is effective in extending the pluripotency of hematopoietic stem cells (HSCs) by antagonizing the arylhydrocarbon receptor, with an IC50 of 127 nM. SteMRegenin 1 is reactive towards human, monkey, and canine species but not rat or murine. The compound has a dose-dependent and reversible effect on CD34-negative cells, enhancing proliferation at low doses and repressing it at high doses.

Uses

Used in Hematopoietic Stem Cell Expansion:
SteMRegenin 1 is used as an AhR antagonist for the expansion and maintenance of hematopoietic stem cells (HSCs) in long-term proliferating/expanding cultures. It enhances and sustains the CD34+ population in serum-free media containing various growth factors, such as Tpo, SCF, Flt3 Ligand, and IL-6.
Used in Immuno-oncology Studies:
SteMRegenin 1 is used as a tool for generating progenitor T cells and natural killer (NK) cells from hematopoietic stem and progenitor cells (HSPCs). These cells can be utilized in immuno-oncology research to study the immune response against cancer and develop novel therapeutic strategies.
Used in Drug Discovery and Development:
SteMRegenin 1 serves as a valuable compound in the development of new drugs targeting the arylhydrocarbon receptor. Its high affinity and selectivity for AhR make it a promising candidate for further research and potential therapeutic applications in various diseases, including cancer and immune disorders.

References

1) Boitano?et al.?(2010),?Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells; Science,?329?1354 2) Tao?et al.?(2017),?StemRegenin 1 selectively promotes expansion of Multipotent Hematopoietic Progenitors derived from Human Embryonic Stem Cells; J. Stem Cells Regen. Med.,?13?75 3) Singh?et al.?(2019),?Generation and function of progenitor T cells from stemRegenin-1-expanded CD34+ human hematopoietic progenitor cells;?Blood Adv,?3?2934 4) Hoogstad-van Evert?et al.?(2017),?Umbilical cord blood CD34+ progenitor-derived NK cells efficiently kill ovarian cancer spheroids and intraperitoneal tumors in NOD/SCID/IL2Rgnull?mice; Oncoimmunology,?6(8)?e1320630

Relevant articles and documents

Methods of Culturing and Expanding Mesenchymal Stem Cells and Isolated Cell Populations Generated Thereby

A method of culturing mesenchymal stem cells (MSCs) is provided. The method comprising culturing a population of the MSCs in a medium comprising an aryl hydrocarbon receptor antagonist, thereby culturing MSCs.