124387-19-5 Usage
Description
5(6)-CARBOXYFLUORESCEIN DIACETATE (5(6)-CFDA) is a cell-permeable, amine-reactive probe that serves as a sensitive, fluorescent substrate for measuring esterase activity in live cells. It is widely utilized in various applications due to its ability to penetrate cell membranes and react with amines, making it a valuable tool in cell biology and related fields.
Uses
Used in Cell Viability Assays:
5(6)-CARBOXYFLUORESCEIN DIACETATE is used as a sensitive substrate for detecting esterase activity in live cells, which is indicative of cell viability. This application is particularly useful in automated cell viability assays, where the fluorescence emitted by the probe can be easily measured and quantified.
Used in "Live-Dead" Assay Systems:
In the context of "Live-Dead" assays, 5(6)-CFDA is employed as a vital dye that can penetrate live cells and be processed by their esterases, resulting in the release of the fluorescent molecule 5(6)-carboxyfluorescein. This allows for the differentiation between live and dead cells based on their fluorescence properties.
Used in Esterase Activity Measurement:
5(6)-CARBOXYFLUORESCEIN DIACETATE is used as a substrate for measuring esterase activity in various applications, such as enzyme assays and drug metabolism studies. The probe's cell permeability and amine reactivity make it an ideal choice for these purposes, as it can provide accurate and reliable measurements of esterase activity in live cells.
Used in Drug Delivery and Release Studies:
Due to its cell permeability and fluorescence properties, 5(6)-CFDA can be used in drug delivery and release studies to monitor the release of drugs from carriers and their subsequent cellular uptake. This application can help researchers optimize drug delivery systems and improve the efficacy of therapeutic agents.
Used in Environmental Toxicity Testing:
5(6)-CARBOXYFLUORESCEIN DIACETATE can also be employed in environmental toxicity testing, where it can be used to assess the impact of pollutants and toxins on cell viability and esterase activity. This information can be valuable for understanding the potential hazards of environmental contaminants and developing strategies to mitigate their effects.
Check Digit Verification of cas no
The CAS Registry Mumber 124387-19-5 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,4,3,8 and 7 respectively; the second part has 2 digits, 1 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 124387-19:
(8*1)+(7*2)+(6*4)+(5*3)+(4*8)+(3*7)+(2*1)+(1*9)=125
125 % 10 = 5
So 124387-19-5 is a valid CAS Registry Number.
InChI:InChI=1/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)
