130539-03-6Relevant articles and documents
Comparative metabolomics and structural characterizations illuminate colibactin pathway-dependent small molecules
Vizcaino, Maria I.,Engel, Philipp,Trautman, Eric,Crawford, Jason M.
, p. 9244 - 9247 (2014)
The gene cluster responsible for synthesis of the unknown molecule colibactin has been identified in mutualistic and pathogenic Escherichia coli. The pathway endows its producer with a long-term persistence phenotype in the human bowel, a probiotic activity used in the treatment of ulcerative colitis, and a carcinogenic activity under host inflammatory conditions. To date, functional small molecules from this pathway have not been reported. Here we implemented a comparative metabolomics and targeted structural network analyses approach to identify a catalog of small molecules dependent on the colibactin pathway from the meningitis isolate E. coli IHE3034 and the probiotic E. coli Nissle 1917. The structures of 10 pathway-dependent small molecules are proposed based on structural characterizations and network relationships. The network will provide a roadmap for the structural and functional elucidation of a variety of other small molecules encoded by the pathway. From the characterized small molecule set, in vitro bacterial growth inhibitory and mammalian CNS receptor antagonist activities are presented.
Peptide nucleic acids (PNAs) with a functional backbone
Pueschl, Ask,Sforza, Stefano,Haaima, Gerald,Dahl, Otto,Nielsen, Peter E.
, p. 4707 - 4710 (2007/10/03)
The synthesis of 10 new T-PNA monomers derived from L-amino acids is presented. The monomers were incorporated into decameric PNA oligomers, and the hybridisation with RNA, DNA and PNA complements studied by thermal stability measurements.
