130727-48-9Relevant articles and documents
Imaging the binding ability of proteins immobilized on surfaces with different orientations by using liquid crystals
Luk, Yan-Yeung,Tingey, Matthew L.,Dickson, Kimberly A.,Raines, Ronald T.,Abbott, Nicholas L.
, p. 9024 - 9032 (2004)
We report an investigation of the binding ability of a protein immobilized on surfaces with different orientations but in identical interfacial microenvironments. The surfaces present mixed self-assembled monolayers (SAMs) of 11 -[19-carboxymethylhexa(ethylene glycol)]undecyl-1-thiol, 1, and 11-tetra(ethylene glycol) undecyl-1-thiol, 2. Whereas 2 is used to define an interfacial microenvironment that prevents nonspecific adsorption of proteins, 1 was activated by two different schemes to immobilize ribonuclease A (RNase A) in either a preferred orientation or random orientations. The binding of the ribonuclease inhibitor protein (RI) to RNase A on these surfaces was characterized by using ellipsometry and the orientational behavior of liquid crystals. Ellipsometric measurements indicate identical extents of immobilization of RNase A via the two schemes. Following incubation of both surfaces with RI, however, ellipsometric measurements indicate a 4-fold higher binding ability of the RNase A immobilized with a preferred orientation over RNase A immobilized with a random orientation. The higher binding ability of the oriented RNase A over the randomly oriented RNase A was also apparent in the orientational behavior of nematic liquid crystals of 4-cyano-4′- pentylcyanobiphenyl (5CB) overlayed on these surfaces. These results demonstrate that the orientations of proteins covalently immobilized in controlled interfacial microenvironments can influence the binding activities of the immobilized proteins. Results reported in this article also demonstrate that the orientational states of proteins immobilized at surfaces can be distinguished by examining the optical appearances of liquid crystals.
Facile method for development of ligand-patterned substrates induced by a chemical reaction
Seo, Hyunjung,Choi, Inseong,Lee, Jeongwook,Kim, Sohyun,Kim, Dong-Eun,Kim, Sang Kyung,Yeo, Woon-Seok
supporting information; experimental part, p. 5804 - 5807 (2011/06/27)
Choose a pattern: A simple and efficient method for ligand patterning on a surface is reported. An organic chemical reaction induces various patterns of an amine functional group, which is further conjugated to a cell adhesion ligand to result in cell patterning (see figure). Copyright
A new glycosylation method. Part 2: Study of carbohydrate elongation onto the gold nanoparticles in a colloidal phase
Shimizu, Hiroki,Sakamoto, Masahiro,Nagahori, Noriko,Nishimura, Shin-Ichiro
, p. 2418 - 2425 (2007/10/03)
A new reaction for carbohydrate elongation for synthesis of oligosaccharide using gold colloidal nanoparticles (GCNPs) has been developed. The gold core in this colloidal phase synthesis was prepared by a reduction of tetrachloroauric acid with 30,31-dithia-3,6,9,12,15,18,43,46,49,52,55,58-dodecaoxa-1,60-hexacontanediol. The presented alkanethionyl oligomeric ethylene glycol worked as a stabilizer of GCNPs and as a linker in chemical elongations of carbohydrates. This colloidal phase synthesis has several advantages such as (1) remnants of reagents and glycosyl donors in each reaction could be easily removed by ultrafiltration or gel filtration column chromatography, (2) further purifications are not required, and (3) the reactions can be monitored by MALDI-TOF MS directly without any pretreatment. In fact, we have successfully synthesized lactose derivative on GCNPs and will report these results in this paper.
