1312674-51-3Relevant articles and documents
Facile synthesis of hydroxymethylcytosine-containing oligonucleotides and their reactivity upon osmium oxidation
Sugizaki, Kaori,Ikeda, Shuji,Yanagisawa, Hiroyuki,Okamoto, Akimitsu
, p. 4176 - 4181 (2011)
DNA strands containing a 5-hydroxymethylcytosine (hmC), which have recently been found in neuron cells and embryonic stem cells, were synthesized through a facile synthetic technique. The hmC-containing strands were efficiently oxidized at hmC using an osmium oxidation assay. The hmC was oxidized as easily as 5-methylcytosine, which can be distinguished from unmethylated cytosine.