1321-34-2Relevant articles and documents
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Wear
, p. 2390 (1951)
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Complete Switch of Reaction Specificity of an Aldolase by Directed Evolution In Vitro: Synthesis of Generic Aliphatic Aldol Products
Junker, Sebastian,Roldan, Raquel,Joosten, Henk-Jan,Clapés, Pere,Fessner, Wolf-Dieter
supporting information, p. 10153 - 10157 (2018/07/31)
A structure-guided engineering of fructose-6-phosphate aldolase was performed to expand its substrate promiscuity toward aliphatic nucleophiles, that is, unsubstituted alkanones and alkanals. A “smart” combinatorial library was created targeting residues D6, T26, and N28, which form a binding pocket around the nucleophilic carbon atom. Double-selectivity screening was executed by high-performance TLC that allowed simultaneous determination of total activity as well as a preference for acetone versus propanal as competing nucleophiles. D6 turned out to be the key residue that enabled activity with non-hydroxylated nucleophiles. Altogether 25 single- and double-site variants (D6X and D6X/T26X) were discovered that show useful synthetic activity and a varying preference for ketone or aldehyde as the aldol nucleophiles. Remarkably, all of the novel variants had completely lost their native activity for cleavage of fructose 6-phosphate.
Thermal decomposition of diethylketone cyclic triperoxide in polar solvents
Barreto, Gaston P.,Alvarez, Elida E.,Eyler, Gladys N.,Canizo, Adriana I.,Allegretti, Patricia E.
, p. 881 - 886 (2014/07/07)
The thermolysis of diethylketone cyclic triperoxide (3,3,6,6,9,9-hexaethyl- 1,2,4,5,7,8-hexaoxacyclononane, DEKTP) was studied in different polar solvents (ethanol, 2-propanol, 1-butanol, 2-butanol, 2-methyl-2-propanol, and acetonitrile). The rate constant values (kd) are higher for reactions performed in secondary alcohols probably because of the possibility to form a cyclic adduct with the participation of the hydrogen atom bonded to the secondary carbon. The kinetic parameters were correlated with the physicochemical properties of the selected solvents. The products of the DEKTP thermal decomposition in different polar solvents support a radical-based decomposition mechanism. CSIRO 2014.