1335224-06-0Relevant articles and documents
Photolabile thymidine cleavable with a 532 nanometer laser
Pickens, Chad J.,Gee, Kyle R.
, p. 4989 - 4991 (2011/10/07)
The synthesis and characterization of a novel 3′ p-hydroxyphenacyl- caged thymidine bearing a 5′ N,N-diisopropylcyanoethylphosphoramidite is presented representing a new methodology for the photoregulation of PCR and gene expression. Solid phase oligonucleotide synthesis affords a primer blocked at the 3′ position, which could function as a phototrigger for polymerase activity. The caging group exhibits quantitative photolysis in 15 s using a 532 nm green hand laser.