13395-35-2 Usage
Description
2-Iminobiotin hydrobromide, also known as 2-Iminobiotin, is a cyclic guanidino analog of biotin that exhibits high affinity for avidin at high pH (>9) and low affinity at low pH (<6). It is characterized by its ability to reversibly inhibit nitric oxide synthases (NOS) through its guanidino group, which plays a role in understanding the binding-site interactions for this class of enzymes.
Uses
Used in Biochemical Research:
2-Iminobiotin hydrobromide is used as a competitive inhibitor for binding to biotin binding sites on streptavidin to restore fluorescence signal. This application is particularly useful in the study of cell surface proteins by labeling and selectively recovering periodate-sensitive components from intact human erythrocyte surfaces.
Used in Enzyme Inhibition:
2-Iminobiotin hydrobromide serves as an inhibitor for nitric oxide synthases (NOS) by reversibly binding to the enzyme via its guanidino group. This inhibition of NOS is crucial for understanding the biosynthesis of nitric oxide and the binding-site interactions of this important class of enzymes. NOS catalyzes the oxidation of the guanidino-nitrogen of L-arginine to produce nitric oxide and L-citrulline, which are essential for various biological processes.
Check Digit Verification of cas no
The CAS Registry Mumber 13395-35-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,3,3,9 and 5 respectively; the second part has 2 digits, 3 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 13395-35:
(7*1)+(6*3)+(5*3)+(4*9)+(3*5)+(2*3)+(1*5)=102
102 % 10 = 2
So 13395-35-2 is a valid CAS Registry Number.
InChI:InChI=1/C10H17N3O2S/c11-10-12-6-5-16-7(9(6)13-10)3-1-2-4-8(14)15/h6-7,9H,1-5H2,(H,14,15)(H3,11,12,13)/t6-,7-,9-/m0/s1
