135682-47-2

Basic information

• Product Name: N-3-carboxy-2-propenoylphenylalanine p-nitroanilide
• CAS NO: 135682-47-2
• Molecular Weight: 383.36
• Mol File: 135682-47-2.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: N-3-carboxy-2-propenoylphenylalanine p-nitroanilide(CAS DataBase Reference)
• NIST Chemistry Reference: N-3-carboxy-2-propenoylphenylalanine p-nitroanilide(135682-47-2)
• EPA Substance Registry System: N-3-carboxy-2-propenoylphenylalanine p-nitroanilide(135682-47-2)

Safety Data

• Hazardous Substances Data: 135682-47-2(Hazardous Substances Data)

Upstream product

• 108-31-6 maleic anhydride 
• 2360-97-6 L-phenylalanine-p-nitroanilide 

Relevant articles and documents

N-ACYLATED PHENYLALANINE p-NITROANILIDES: NEW SUBSTRATES FOR CHYMOTRYPSIN

Chromogenic substrates of the following types were synthesized: X-Phenylalanine p-nitroanilides, where X = acetyl, hydroxyacetyl, methoxyethoxyacetyl, and 3-carboxy-2-propenoyl, (glycyl)n-phenylalanine p-nitroanilides and 3-carboxypropanoyl(glycyl)n-phenylalanine p-nitroanilides, where n= 1,2,3, (glycyl)n-tyrosine p-nitroanilides and 3-carboxypropanoyl(glycyl)n-tyrosine p-nitroanilides, where n= 0,1,2, and also acetylhistidyl-glycyl-glycyl-phenylalanine p-nitroanilide.The konstants Km, kcat and C (i.e. kcat/Km) were estimated for enzymatic splitting by chymotrypsin.The greatest kcat values were found for 3-carboxypropanoyl-glycyl-glycyl-tyrosine p-nitroanilide and 3-carboxypropanoyl-glycyl-glycyl-phenylalanine p-nitroanilide, 40.5 and 16.9, respectively.The smallest Km value, 1.1E-04 (mM) was observed with acetylhistidyl-glycyl-glycyl-phenylalanine p-nitroanilide, which exhibited also the greatest C constant, 115 000 (M-1s-1).