136112-90-8Relevant articles and documents
Incorporation of methylated pyrimidine analogues into RNA
Vyle, Joseph S.,Young, Karen J.,Grasby, Jane A.
, p. 5093 - 5096 (1998)
Routes for the preparation of 2'-silyl protected phosphoramidites of the modified nucleosides O2-methyluridine and O4-methyluridine are described. Methodology for the site-specific incorporation of these phosphonamidetes into a 25 nucleotide long oligoribonucleotide sequence by solid-phase synthesis is reported.
24. Synthesis of (15N2)[17O]urea, (15N2)[O2,O4-17O 2]uridine, and (15N3)[O2-17O]cytidine
Amantea, Antonio,Henz, Matthias,Strazewski, Peter
, p. 244 - 254 (1996)
A general synthetic approach for the synthesis of 15N- and 17O-doubly labelled pyrimidine nucleosides is described. The 15N isotopes in uridine and the 17O isotope in the urea-derived carbonyl group of uridine and cytidine originate from (15N2)[17O]urea (5) which was synthesized from 15NH4Cl, thiophosgene (1), and H2[17O]. The third 15N isotope of cytidine in 4-position stems from the substitution of the 1,2,4-triazole moiety of (15N2)[O2-17O]uridine derivative 8a/b with 15NH4OH. Hydrolysis of the same key intermediate 8a/b with Na[17O]H/H2[17O] introduced the second 17O isotope into the 4-position of uridine. The 15N- and 17O-NMR spectra of the target compounds 12 and 14 in phosphate-buffered H2O serve as references for heteronuclear NMR spectra of labelled RNA fragments.