138182-21-5 Usage
Description
6-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE, commonly known as X-Gal, is a synthetic compound that serves as a substrate for the enzyme β-galactosidase. It is characterized by its powder form and is widely utilized in various applications due to its ability to detect enzyme activity and its role as a pharmaceutical intermediate.
Uses
Used in Enzyme Activity Detection:
6-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE is used as an enzyme substrate for β-galactosidase activity detection in lac+ bacterial colonies. It acts as an alternative to X-Gal, providing a means to identify and differentiate between recombinant and non-recombinant colonies through a colorimetric assay.
Used in Pharmaceutical Industry:
6-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE is used as a pharmaceutical intermediate to reduce toxicity and improve the efficacy of certain medications. Its role in this industry is crucial for the development of safer and more effective drugs.
Used in Diagnostic Reagents:
In the field of diagnostics, 6-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE serves as an enzyme substrate in diagnostic reagents. It is particularly useful in conjunction with IPTG for the detection of β-galactosidase activity in bacterial colonies, allowing for the identification of recombinants from non-recombinants through a colorimetric assay.
Used in Research and Development:
6-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE is also employed in research and development for the simultaneous detection of GUS and Lac activities on the same plate when used in conjunction with X-glu. This application aids scientists in their studies and experiments, contributing to the advancement of various fields.
General Information:
The product is available in different package sizes, including 25 mg, 100 mg, and 1 gm containers, catering to the needs of various users and applications.
Check Digit Verification of cas no
The CAS Registry Mumber 138182-21-5 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,3,8,1,8 and 2 respectively; the second part has 2 digits, 2 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 138182-21:
(8*1)+(7*3)+(6*8)+(5*1)+(4*8)+(3*2)+(2*2)+(1*1)=125
125 % 10 = 5
So 138182-21-5 is a valid CAS Registry Number.
