1386385-76-7 Usage
Description
N-(3-azidoprop-1-yl)fluoresceinyl-6-carboxamide, also known as FAM (fluorescein) azide, is a Click Chemistry labeling reagent derived from the fluorescein family. It is the pure 6-isomer and is known for its absorption at 488nm, making it a popular dye in cell biology. FAM (fluorescein) azide serves as an alternative to other dyes such as Alexa Fluor 488 and DyLight 488 due to its unique properties and applications.
Uses
Used in Cell Biology:
N-(3-azidoprop-1-yl)fluoresceinyl-6-carboxamide is used as a fluorescent dye for cell biology applications. It is particularly useful for the fluorescent labeling of terminal alkyne-modified molecules through the Cu(I)-catalyzed azide-alkyne (CuAAC) reaction, also known as the click reaction. This reaction allows for the specific and efficient labeling of target molecules, enabling researchers to study various cellular processes and interactions with high precision.
Used in Biomedical Research:
In the field of biomedical research, N-(3-azidoprop-1-yl)fluoresceinyl-6-carboxamide is employed as a versatile tool for the detection and analysis of biological molecules. Its ability to label molecules with high specificity and sensitivity makes it an invaluable asset in the study of molecular interactions, cellular signaling pathways, and the development of targeted drug delivery systems.
Used in Drug Development:
N-(3-azidoprop-1-yl)fluoresceinyl-6-carboxamide is also utilized in drug development as a labeling agent for the identification and tracking of drug candidates. Its fluorescent properties allow researchers to monitor the distribution, metabolism, and excretion of drugs within biological systems, providing valuable insights into the pharmacokinetics and pharmacodynamics of potential therapeutic agents.
Used in Diagnostic Applications:
In the diagnostic industry, N-(3-azidoprop-1-yl)fluoresceinyl-6-carboxamide is used as a component in the development of diagnostic assays and tests. Its fluorescent labeling capabilities enable the detection and quantification of specific biomarkers, pathogens, or other target molecules, contributing to the early and accurate diagnosis of various diseases and conditions.
Used in Imaging Techniques:
N-(3-azidoprop-1-yl)fluoresceinyl-6-carboxamide is employed in various imaging techniques, such as fluorescence microscopy and flow cytometry, to visualize and analyze cellular structures, functions, and interactions. Its bright fluorescence and compatibility with common imaging equipment make it a preferred choice for researchers and clinicians alike.
Check Digit Verification of cas no
The CAS Registry Mumber 1386385-76-7 includes 10 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 7 digits, 1,3,8,6,3,8 and 5 respectively; the second part has 2 digits, 7 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 1386385-76:
(9*1)+(8*3)+(7*8)+(6*6)+(5*3)+(4*8)+(3*5)+(2*7)+(1*6)=207
207 % 10 = 7
So 1386385-76-7 is a valid CAS Registry Number.
1386385-76-7Relevant articles and documents
Benzoylphosphonate-based photoactive phosphopeptide mimetics for modulation of protein tyrosine phosphatases and highly specific labeling of SH2 domains
Horatscheck, André,Wagner, Stefan,Ortwein, Jutta,Kim, Boo Geun,Lisurek, Michael,Beligny, Samuel,Schütz, Anja,Rademann, J?rg
, p. 9441 - 9447 (2012/10/29)
A light switch for phosphotyrosine- recognizing proteins: Irradiation of the bioisosteric benzoylphosphonate suffices to "turn off" the activity of target proteins and to label them covalently (see scheme). Photoactive bioisosters may find applications in functional cell biology, bioanalytics, and proteome research.
