13927-71-4Relevant articles and documents
Copper diethyldithiocarbamate as an activator of Nrf2 in cultured vascular endothelial cells
Fujie, Tomoya,Murakami, Masaki,Yoshida, Eiko,Tachinami, Tadashi,Shinkai, Yasuhiro,Fujiwara, Yasuyuki,Yamamoto, Chika,Kumagai, Yoshito,Naka, Hiroshi,Kaji, Toshiyuki
, p. 263 - 273 (2016/03/30)
The interest in organic-inorganic hybrid molecules as molecular probes for biological systems has been growing rapidly. Such hybrid molecules exhibit unique biological activities. Herein, copper(II) bis(diethyldithiocarbamate) (Cu10) was found to activate the transcription factor NF-E2-related factor 2 (Nrf2), which is responsible for regulating antioxidant and phase II xenobiotic enzymes, in vascular endothelial cells. The copper complex rapidly accumulated within cells and induced nuclear translocation of Nrf2, leading to upregulation of the expression of downstream proteins without cytotoxic effects. However, while copper bis(2-hydroxyethyl)dithiocarbamate activated Nrf2, copper ion, diethyldithiocarbamate ligand with or without zinc or iron failed to exhibit this activity. Intracellular accumulation of Cu10 was higher than that of Cu(II) and Cu(I). While the accumulation of copper(II) bis(dimethyldithiocarbamate) was reduced by small interfering RNA (siRNA)-mediated knockdown of the copper transporter CTR1, the knockdown did not affect Cu10 accumulation, indicating that Cu10 rapidly enters vascular endothelial cells via CTR1-independent mechanisms. In addition, copper and iron complexes with other ligands tested could not activate Nrf2, suggesting that the intramolecular interaction between copper and dithiocarbamate ligand is important for the activation of the transcription factor. Cu10 induced the expression of heme oxygenase-1, NAD(P)H quinone oxidoreductase 1, and γ-glutamylcysteine synthetase, downstream proteins of Nrf2. It was suggested that Cu10-induced activation of Nrf2 was due to proteasome inhibition as well as binding to Kelch-like ECH-associated protein 1. Since the effects of Cu10 on vascular endothelial cells are unique and diverse, the copper complex may be a good molecular probe to analyze the functions of the cells.
Electron Spin Resonance Study of Some Copper(II) Dithiocarbamates and their Mixed-ligand Complexes
Newton, William J.,Tabner, Brian J.
, p. 466 - 471 (2007/10/02)
The e.s.r. spectra of seven copper(II) dithiocarbamates have been recorded in chloroform-ethanol mixtures at room temperature and at 113 K.It has been found that the addition of a range of salts to these dithiocarbamates destroys the complex forming a mixed-ligand complex, , with a new e.s.r. spectrum.When X is Cl or Br the additional interaction of the unpaired electron with a single halogen nucleus is observed.The complex forms a dipole-dipole coupled dimer and a computer simulation of the ΔMs = 2 absorption of this dimer has been obtained.
