141223-71-4

Basic information

• Product Name: BENZYLOXYCARBONYL-L-VALYL-L-LYSYL-L-METHIONINE 4-METHYLCOUMARYL-7-AMIDE HYDROCHLORIDE
• Synonyms: PROTEASOME SUBSTRATE IV (FLUOROGENIC);N-CBZ-VAL-LYS-MET 7-AMIDO-4-METHYLCOUMARIN;Z-VKM-AMC;Z-VAL-LYS-MET-AMC;Z-VAL-LYS-MET-AMC ACETATE SALT;Z-VAL-LYS-MET-MCA HYDROCHLORIDE;BENZYLOXYCARBONYL-L-VALYL-L-LYSYL-L-METHIONINE 4-METHYLCOUMARYL-7-AMIDE HYDROCHLORIDE;z-val-lys-met 7-amido-4-methylcoumarin
• CAS NO: 141223-71-4
• Molecular Formula: C34H45N5O7S
• Molecular Weight: 667.82
• Product Categories: Amyloid A4-generating enzymes;Amyloid A4-generating enzymesEnzyme Substrates;Enzyme Substrates;Protease Substrates;Substrates by Enzyme
• Mol File: 141223-71-4.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 966.2±65.0 °C(Predicted)
• Appearance: /
• Density: 1.245±0.06 g/cm3(Predicted)
• Storage Temp.: −20°C
• PKA: 11.05±0.46(Predicted)
• CAS DataBase Reference: BENZYLOXYCARBONYL-L-VALYL-L-LYSYL-L-METHIONINE 4-METHYLCOUMARYL-7-AMIDE HYDROCHLORIDE(CAS DataBase Reference)
• NIST Chemistry Reference: BENZYLOXYCARBONYL-L-VALYL-L-LYSYL-L-METHIONINE 4-METHYLCOUMARYL-7-AMIDE HYDROCHLORIDE(141223-71-4)
• EPA Substance Registry System: BENZYLOXYCARBONYL-L-VALYL-L-LYSYL-L-METHIONINE 4-METHYLCOUMARYL-7-AMIDE HYDROCHLORIDE(141223-71-4)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 141223-71-4(Hazardous Substances Data)

Usage

Description

BENZYLOXYCARBONYL-L-VALYL-L-LYSYL-L-METHIONINE 4-METHYLCOUMARYL-7-AMIDE HYDROCHLORIDE is a complex peptide derivative with a unique structure, characterized by its benzyloxycarbonyl group, valyl and lysyl amino acid residues, and a 4-methylcoumaryl-7-amide moiety. BENZYLOXYCARBONYL-L-VALYL-L-LYSYL-L-METHIONINE 4-METHYLCOUMARYL-7-AMIDE HYDROCHLORIDE is synthesized as a fluorogenic substrate, designed to be hydrolyzed by specific enzymes, leading to the release of a fluorescent product upon cleavage.

Uses

Used in Research and Diagnostic Applications:
BENZYLOXYCARBONYL-L-VALYL-L-LYSYL-L-METHIONINE 4-METHYLCOUMARYL-7-AMIDE HYDROCHLORIDE is used as a fluorogenic proteasome substrate for measuring the peptidase activity of the 20S proteasome. This application is crucial in understanding the role of proteasomes in cellular processes and their involvement in various diseases, including cancer and neurodegenerative disorders.
Used in Alzheimer's Disease Research:
In the field of Alzheimer's disease research, BENZYLOXYCARBONYL-L-VALYL-L-LYSYL-L-METHIONINE 4-METHYLCOUMARYL-7-AMIDE HYDROCHLORIDE serves as a fluorogenic substrate for amyloid A4-generating enzymes. The compound aids in studying the mechanisms behind amyloid-beta peptide production, which is a key factor in the development of Alzheimer's disease.
The compound's fluorescence properties, with an excitation maximum of 340-360nm and an emission maximum of 440-460nm, make it a valuable tool for detecting and monitoring enzyme activity in various experimental setups. Its use in research and diagnostics contributes to the advancement of knowledge in proteasome function and the development of potential therapeutic strategies for related diseases.