1419234-19-7Relevant articles and documents
Efficient N-terminal labeling of proteins by use of sortase
Williamson, Daniel J.,Fascione, Martin A.,Webb, Michael E.,Turnbull, W. Bruce
supporting information, p. 9377 - 9380 (2012/11/06)
"Sorting out" N-terminal labeling: The reversibility of transpeptidase reactions makes protein N-terminal labeling challenging. Depsipeptide substrates for sortase A release alcohol by-products, which are poor nucleophiles for the reverse reaction, during ligation. Proteins with an unhindered N-terminal glycine residue can be labeled efficiently with only a minimal excess of the labeling reagent (see scheme).