1441046-64-5Relevant articles and documents
BODIPY-Coumarin Conjugate as an Endoplasmic Reticulum Membrane Fluidity Sensor and Its Application to ER Stress Models
Lee, Hoyeon,Yang, Zhigang,Wi, Youngjin,Kim, Tae Woo,Verwilst, Peter,Lee, Yun Hak,Han, Ga-In,Kang, Chulhun,Kim, Jong Seung
, p. 2474 - 2480 (2015)
An endoplasmic reticulum (ER) membrane-selective chemosensor composed of BODIPY and coumarin moieties and a long alkyl chain (n-C18) was synthesized. The emission ratio of BODIPY to coumarin depends on the solution viscosity. The probe is localized to the ER membrane and was applied to reveal the reduced ER membrane fluidity under ER stress conditions.
A self-calibrating bipartite viscosity sensor for mitochondria
Yang, Zhigang,He, Yanxia,Lee, Jae-Hong,Park, Nayoung,Suh, Myungkoo,Chae, Weon-Sik,Cao, Jianfang,Peng, Xiaojun,Jung, Hyosung,Kang, Chulhun,Kim, Jong Seung
, p. 9181 - 9185 (2013)
A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.
