145224-94-8 Usage
Description
MES Monohydrate, also known as 2-(N-morpholino)ethanesulfonic acid monohydrate, is a zwitterionic buffer that is not absorbed through cell membranes and is virtually transparent in UV light. It is a buffering agent used in many biological and biochemical applications, including cell culture media, protein-based buffer formulations, and purification processes. The characteristics of low UV absorptivity, minimal reactivity, stable pH, and solubility in water make MES Monohydrate an ideal Good's buffer for various research and industrial applications.
Uses
Used in Cell Culture Media:
MES monohydrate is used as a buffering agent to maintain a stable environment in cell culture media for various cell types, including bacteria, yeast, and mammalian cells. It helps to keep the pH at a nearly constant value, which is crucial for cell growth and function.
Used in Enzymatic Solutions:
As a buffering agent, MES monohydrate is used to stabilize enzymatic solutions, ensuring that the enzymes maintain their activity and function over time.
Used in Electrophoresis:
MES monohydrate serves as a running buffer in polyacrylamide gel electrophoresis (PAGE) for the separation of proteins and nucleic acids. Its low ionic mobility makes it an excellent buffer for use in capillary electrochromatography.
Used in Protein Purification:
In the bioprocesses of antibody, peptide, and protein purification, as well as the purification of blood components and growth factors, MES monohydrate is used as a buffering agent to maintain a stable pH environment during the purification process.
Used in Anticancer Applications:
Although not explicitly mentioned in the provided materials, MES monohydrate has been reported to complex with some organotin(IV) molecules that are being explored as antitumor agents, potentially contributing to its use in cancer research and treatment.
Used in Biological and Biochemical Techniques:
MES Monohydrate is used in various biological and biochemical techniques where highly purified products are needed, such as in vitro cell wall stress assays and as a component of PAGE running buffer.
Preparation and handling
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Precautions
Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.
Check Digit Verification of cas no
The CAS Registry Mumber 145224-94-8 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,5,2,2 and 4 respectively; the second part has 2 digits, 9 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 145224-94:
(8*1)+(7*4)+(6*5)+(5*2)+(4*2)+(3*4)+(2*9)+(1*4)=118
118 % 10 = 8
So 145224-94-8 is a valid CAS Registry Number.
