148608-53-1

Basic information

• Product Name: 5-formylcytidine
• Synonyms: 5-formylcytidine
• CAS NO: 148608-53-1
• Molecular Formula: C10H13 N3 O6
• Molecular Weight: 271.23
• Mol File: 148608-53-1.mol
• Article Data: 4

Chemical Properties

• Boiling Point: 594.9°Cat760mmHg
• Flash Point: 313.6°C
• Appearance: /
• Density: 1.91g/cm³
• Vapor Pressure: 1.24E-16mmHg at 25°C
• Refractive Index: 1.755
• Storage Temp.: Hygroscopic, -20°C Freezer, Under inert atmosphere
• Solubility: DMSO (Slightly, Sonicated), Methanol (Slightly, Heated), Water (Slightly, Heated
• PKA: 13.48±0.70(Predicted)
• Stability: Hygroscopic
• CAS DataBase Reference: 5-formylcytidine(CAS DataBase Reference)
• NIST Chemistry Reference: 5-formylcytidine(148608-53-1)
• EPA Substance Registry System: 5-formylcytidine(148608-53-1)

Safety Data

• Hazardous Substances Data: 148608-53-1(Hazardous Substances Data)

Usage

Description

5-Formylcytidine is a modified nucleoside that is specifically located at the first position of the anticodon in bovine mitochondrial tRNAMet. This unique positioning and modification play a crucial role in the proper functioning of the tRNA and, by extension, the overall process of protein synthesis within the mitochondria.

Uses

Used in Molecular Biology and Genetics Research:
5-Formylcytidine is used as a key molecular marker for studying the structure and function of tRNA, particularly in the context of mitochondrial translation. Its presence in the anticodon loop of tRNAMet provides valuable insights into the mechanisms of genetic code reading and the role of modified nucleosides in ensuring accurate translation.
Used in Drug Development:
5-Formylcytidine can be utilized as a target for the development of novel therapeutic agents that modulate mitochondrial function. Given its specific role in tRNAMet, compounds that interact with or affect the function of 5-formylcytidine could potentially be used to treat diseases associated with mitochondrial dysfunction, such as neurodegenerative disorders and metabolic diseases.
Used in Diagnostics:
The presence of 5-formylcytidine in mitochondrial tRNA can be exploited for the development of diagnostic tools to detect and monitor mitochondrial disorders. By measuring the levels or modifications of 5-formylcytidine, clinicians may be able to assess the health of a patient's mitochondria and diagnose conditions related to mitochondrial dysfunction.
Used in Synthetic Biology:
5-Formylcytidine can serve as a building block for the synthesis of modified nucleic acids with novel properties. In the field of synthetic biology, researchers can use this modified nucleoside to create custom-designed RNA or DNA molecules with specific functions, such as enhanced stability, altered binding properties, or novel catalytic activities.
Used in Biochemical Education:
5-Formylcytidine can be employed as a teaching tool to illustrate the importance of modified nucleosides in the context of tRNA structure and function. By studying this specific example, students can gain a deeper understanding of the complex interactions between tRNA, mRNA, and ribosomes during the process of translation, as well as the role of post-transcriptional modifications in regulating cellular processes.

InChI:InChI=1/C10H13N3O6/c11-8-4(2-14)1-13(10(18)12-8)9-7(17)6(16)5(3-15)19-9/h1-2,5-7,9,15-17H,3H2,(H2,11,12,18)/t5-,6-,7-,9-/m1/s1

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Relevant articles and documents

The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

We developed a novel strategy by oxidation-derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.

SYNTHESIS AND STRUCTURE OF HIGH POTENCY RNA THERAPEUTICS

This invention provides expressible polynucleotides, which can express a target protein or polypeptide. Synthetic mRNA constructs for producing a protein or polypeptide can contain one or more 5′ UTRs, where a 5′ UTR may be expressed by a gene of a plant. In some embodiments, a 5′ UTR may be expressed by a gene of a member of Arabidopsis genus. The synthetic mRNA constructs can be used as pharmaceutical agents for expressing a target protein or polypeptide in vivo.