148819-94-7 Usage
Description
CARBOXY-PTIO, also known as 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, is a water-soluble, stable organic free radical that primarily functions as a scavenger of nitric oxide (NO). It reacts stoichiometrically with NO, converting it to NO2 and allowing for the specific investigation of the effects of NO without significantly affecting other NO-related metabolites. This makes CARBOXY-PTIO a valuable tool for studying the role of NO in various physiological processes.
Uses
Used in Research and Development:
CARBOXY-PTIO is used as a nitric oxide (NO) scavenger for investigating the role of NO in physiological processes. It reacts with NO in a 1:1 stoichiometry, producing NO2–/NO3–, which helps researchers understand the involvement of NO in various biological systems.
Used in Electron Paramagnetic Resonance (EPR) Detection:
CARBOXY-PTIO is used as a NO scavenger for EPR detection of NO. This application allows for the specific investigation of the effect of NO in different experimental setups.
Used in Analyzing Hydroxyl Radicals and Nitric Oxide Generation:
CARBOXY-PTIO potassium salt has been utilized to check the generation of hydroxyl radicals or nitric oxide in reaction mixtures. This helps researchers examine the generation of nitric oxide in various reaction mixtures and understand its role in different chemical processes.
Used in Neuronal Studies:
CARBOXY-PTIO has been added to neurons to analyze its effect on glucose/oxygen/serum deprivation (GOSD) conditions. This application aids in understanding the role of NO in neuronal function and its potential impact on various neurological conditions.
Used in Endotoxin Shock Models:
In LPS-induced endotoxin shock, CARBOXY-PTIO has been shown to reverse hypotension and renal dysfunction and increase survival in a rat model. This demonstrates its potential use in studying and potentially treating conditions related to endotoxin shock and NO involvement.
Biological Activity
A stable, water-soluble free radical that reacts stoichiometrically with NO. Almost twice as strong an inhibitor as N( ω )-nitroarginine or N-methylarginine.
Biochem/physiol Actions
Carboxy-PTIO can make a quick reaction with nitric oxide?(NO) to produce nitrogen dioxide (NO2). It can be used to prevent hypotension and endotoxic shock, stimulated by lipopolysaccharide in- vivo. Carboxy-PTIO can also block in vitro vascular relaxation, stimulated by NO.
References
Akaike et al. (1993), Antagonistic action of imidazolineoxyl N-oxides against endothelium-derived relaxing factor/.NO through a radical reaction, Biochemistry, 32 827
Yoshida et al. (1994), Therapeutic effects of imidazolineoxyl N-oxide against endotoxin shock through its direct nitric oxide-scavenging activity; Biochem. Biophys. Res. Commun., 202 923
Hada et al. (2019), Nitric Oxide Is Involved in Heavy Ion-Induced Non-Targeted Effects in Human Fibroblasts; Int. J. Mol. Sci., 20 4327
Jin et al. (2020), A novel quinolinylmethyl substituted ethylenediamine compound exerts anti-cancer effects via stimulating the accumulation of reactive oxygen species and NO in hepatocellular carcinoma cells; Eur. J. Pharmacol., 885 173497
Check Digit Verification of cas no
The CAS Registry Mumber 148819-94-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,8,8,1 and 9 respectively; the second part has 2 digits, 9 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 148819-94:
(8*1)+(7*4)+(6*8)+(5*8)+(4*1)+(3*9)+(2*9)+(1*4)=177
177 % 10 = 7
So 148819-94-7 is a valid CAS Registry Number.
InChI:InChI=1/C14H17N2O4.K/c1-13(2)14(3,4)16(20)11(15(13)19)9-5-7-10(8-6-9)12(17)18;/h5-8H,1-4H3,(H,17,18);/q;+1/p-1