14994-67-3

Basic information

• Product Name: 1-Phenazinol 10-oxide
• Synonyms: 1-Phenazinol 10-oxide
• CAS NO: 14994-67-3
• Molecular Formula: C12H8N2O2
• Molecular Weight: 212.2
• Mol File: 14994-67-3.mol
• Article Data: 1

Chemical Properties

• Appearance: /
• CAS DataBase Reference: 1-Phenazinol 10-oxide(CAS DataBase Reference)
• NIST Chemistry Reference: 1-Phenazinol 10-oxide(14994-67-3)
• EPA Substance Registry System: 1-Phenazinol 10-oxide(14994-67-3)

Safety Data

• Hazardous Substances Data: 14994-67-3(Hazardous Substances Data)

Upstream product

• 528-71-2 phenazin-1-ol 

Relevant articles and documents

Biosynthetic Pathway Construction and Production Enhancement of 1-Hydroxyphenazine Derivatives in Pseudomonas chlororaphis H18

1-Hydroxyphenazine derivatives are phenazine family chemicals with broad-spectrum antibacterial and potential biological activities. However, the lack of variety and low titer hinder their applications. In this research, three enzymes PhzS (monooxygenase), NaphzNO1 (N-monooxygenase), and LaphzM (methyltransferase) were heterologously expressed in a phenazine-1-carboxylic acid generating strain Pseudomonas chlororaphis H18. Four phenazines, 1-hydroxyphenazine, 1-methoxyphenazine, 1-hydroxyphenazine N′ 10-oxide, and a novel phenazine derivative 1-methoxyphenazine N′ 10-oxide, were isolated, characterized in the genetically modified strains, and exhibited excellent antimicrobial activities. Next, we verified the hydroxyl methylation activity of LaphzM and elucidated the biosynthetic pathway of 1-methoxyphenazine N′ 10-oxide in vitro. Moreover, the titer of 1-hydroxyphenazine derivatives was engineered. The three compounds 1-methoxyphenazine, 1-hydroxyphenazine N′ 10-oxide, and 1-methoxyphenazine N′ 10-oxide all reach the highest titer reported to date. This work provides a promising platform for phenazine derivatives' combinatorial biosynthesis and engineering.