150849-52-8

Basic information

• Product Name: (2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt
• Synonyms: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monosodiumsalt;5-(2,4-Disulfophenyl)-2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium mon;GLT001;Water-soluble tetrazoliuM -1;sodium 4-(3-(4-iodophenyl)-2-(4-nitrophenyl)-2,3-dihydro-1H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate;(2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt;4-[3-(4-IODOPHENYL)-2-(4-NITROPHENYL)-2H-5-TETRAZOLIO]-1,3-BENZENE DISULFONATE;WST 1
• CAS NO: 150849-52-8
• Molecular Formula: C19H11IN5NaO8S2
• Molecular Weight: 651.34
• EINECS: 999-999-2
• Product Categories: Labeling and Diagnostics Reagents
• Mol File: 150849-52-8.mol
• Article Data: 0

Chemical Properties

• Melting Point: > 300°C (dec.)
• Appearance: pale yellow or brown powder
• Storage Temp.: -20°C Freezer
• Stability: 4°C in the Dark
• CAS DataBase Reference: (2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt(CAS DataBase Reference)
• NIST Chemistry Reference: (2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt(150849-52-8)
• EPA Substance Registry System: (2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt(150849-52-8)

Safety Data

• Hazardous Substances Data: 150849-52-8(Hazardous Substances Data)

Usage

Description

(2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt, also known as WST-1, is a highly sensitive tetrazolium reagent with a light red color. It is used in various biological assays due to its ability to produce a water-soluble formazan with a dark red color upon cleavage by mitochondrial dehydrogenase. WST-1 is a brownish-yellow solid and exhibits a maximum absorbance (l max) of 438nm with an extinction coefficient (e) of 3.7 x 10^4.

Uses

1. Used in Cell Proliferation Assays:
WST-1 is used as a cell proliferation indicator in various cell culture experiments. It measures the activity of mitochondrial dehydrogenase, which is directly related to the number of living cells in a sample. The conversion of WST-1 to formazan is indicative of cell viability and proliferation, making it a valuable tool for assessing the effects of different treatments or conditions on cell growth.
2. Used in NADH Assays:
WST-1 is also employed in assays that measure the activity of NADH, an essential coenzyme involved in cellular respiration and energy production. The conversion of WST-1 to formazan in the presence of NADH provides a quantitative measure of NADH levels, which can be useful in studying metabolic processes and the effects of various compounds on cellular metabolism.
3. Used in Drug Screening and Toxicity Studies:
Due to its sensitivity and ability to measure cell viability, WST-1 is widely used in drug screening and toxicity studies. It helps researchers evaluate the potential cytotoxic effects of new compounds, as well as their impact on cell proliferation and overall cell health.
4. Used in Research on Mitochondrial Function:
WST-1's reliance on mitochondrial dehydrogenase for its conversion to formazan makes it an ideal tool for studying mitochondrial function and dysfunction in various diseases and conditions. It can be used to investigate the effects of different factors on mitochondrial activity and to identify potential therapeutic targets for mitochondrial-related disorders.