156-54-7 Usage
Description
Sodium Butyrate, with the chemical formula Na(C3H7COO), is the sodium salt of butyric acid. It is a white crystalline solid with an unpleasant smell and has various effects on cultured mammalian cells, such as inhibition of proliferation, induction of differentiation, and regulation of gene expression.
Uses
1. Used in Pharmaceutical Applications:
Sodium Butyrate is used as a histone deacetylase (HDAC) inhibitor for the treatment of physiological disorders like Duchenne muscular dystrophy. It also has potential as an antitumor agent when combined with other cytotoxic agents, showing effectiveness in treating carcinomas.
2. Used in Research and Development:
Sodium Butyrate is used as a component in homogenization and immunoprecipitation buffer for HDAC inhibition in fly embryos and insect S2 cells. It is also utilized as an HDAC inhibitor in breast cancer cell line MDA-MB-231, analyzed through cell viability assay and viral replication assay.
3. Used in Bioreactor Applications:
Sodium Butyrate is used to enhance the production of recombinant tissue-plasminogen activator (t-PA) in Chinese hamster ovary (CHO) cells in a bioreactor, improving the efficiency of the production process.
4. Used in Chemical Synthesis:
As an organic sodium salt, Sodium Butyrate can be used in various chemical synthesis processes, taking advantage of its unique properties as a result of the replacement of the proton from the carboxy group of butyric acid by a sodium ion.
Biological Activity
Histone deacetylase inhibitor. Restores contextual memory in a transgenic mouse model of Alzheimer's disease. Shown to induce pancreatic progenitor formation. Directs the differentiation of mouse embryonic stem cells into hepatocytes when used in combination with the cytokine Activin A.
Biochem/physiol Actions
Sodium butyrate is a short-chain fatty acid that inhibits histone deacetylase (HDAC) in the millimolar range. Decreases Ca2+ release from intracellular stores. Induces apoptosis in several cell lines. Sodium butyrate induces the production of recombinant protein in Chinese hamster ovary (CHO) cells and other human cell lines. Sodium butyrate affects the proteome and gene expression pathways in the cells. It represses cell cycle related genes and modifies the genes involved in cell metabolism and apoptosis.
Purification Methods
Sodium butyrate [156-54-7] M 110.1. Prepare it by neutralising the acid with Na2CO3, and recrystallising it from EtOH. [Beilstein 2 IV 779.]
Check Digit Verification of cas no
The CAS Registry Mumber 156-54-7 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 1,5 and 6 respectively; the second part has 2 digits, 5 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 156-54:
(5*1)+(4*5)+(3*6)+(2*5)+(1*4)=57
57 % 10 = 7
So 156-54-7 is a valid CAS Registry Number.
InChI:InChI=1/C4H8O2.Na/c1-2-3-4(5)6;/h2-3H2,1H3,(H,5,6);/q;+1/p-1
156-54-7Relevant articles and documents
Process for preparing hyperpolarized substrates and method for MRI
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Page/Page column 27, (2017/08/01)
The present invention generally relates to a process for the preparation of aqueous solutions of hyperpolarized molecules ready for use in in-vivo MR diagnostic imaging, the use thereof as MRI contrast agent in investigation methods for producing diagnostic MR images of a human or non-human animal body organ, region or tissue.
Enantioselective catabolism of racemic serine: Preparation of d-serine using whole cells of Fusobacterium nucleatum
Ramezani, Mohammad,White, Robert L.
experimental part, p. 1473 - 1478 (2011/11/12)
Incubation of racemic serine with the anaerobic bacterium Fusobacterium nucleatum yielded d-serine in high enantiomeric excess (>95%). Selective degradation of the l-amino acid was most efficient when F. nucleatum was resuspended in a buffer at high cell densities (ca. 50-100 g damp cells/L); a single incubation effectively removed almost all l-serine from racemic mixtures at initial concentrations up to 800 mM, the solubility limit. The product d-amino acid was separated from the metabolic end-products (acetate, butyrate and lactate) and buffer components by a single cation-exchange step. After recrystallization, 83% of the d-serine in the initial racemate was recovered with >99% ee (HPLC) and 98% purity (HPLC). This anaerobic microbial approach provides a viable complementary method for generating d-serine, a valuable chiral starting material for chemical synthesis.
