159680-32-7

Basic information

• Product Name: L-GLUTAMINE (5-13C)
• Synonyms: Aesgen 14-13C;Cebrogen-13C;GluMin-13C;L-2-AMinoglutaraMidic Acid-13C;LevoglutaMide-13C;L-GlutaMic Acid γ-ΑMide-13C;NSC 27421-13C;Saforis-13C
• CAS NO: 159680-32-7
• Molecular Formula: C5H10N2O3
• Molecular Weight: 147.15
• Product Categories: Amino Acids 13C, 2H, 15N;Alphabetical Listings;Amino Acids;Amino AcidsStable Isotopes;G-HStable Isotopes;MRI/MRS;Stable Isotopes;Aliphatics;Amino Acids & Derivatives;Isotope Labelled Compounds
• Mol File: 159680-32-7.mol
• Article Data: 3

Chemical Properties

• Melting Point: 185 °C (dec. )(lit.)
• Appearance: /
• Storage Temp.: -20°C Freezer, Under inert atmosphere
• Solubility: Aqueous Acid (Slightly), Water (Slightly, Sonicated)
• CAS DataBase Reference: L-GLUTAMINE (5-13C)(CAS DataBase Reference)
• NIST Chemistry Reference: L-GLUTAMINE (5-13C)(159680-32-7)
• EPA Substance Registry System: L-GLUTAMINE (5-13C)(159680-32-7)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 159680-32-7(Hazardous Substances Data)

Usage

Description

L-GLUTAMINE (5-13C) is an isotopically labeled form of the essential amino acid L-glutamine, which plays a vital role in various biological processes and serves as a significant energy source for cells in culture. It is known for its stability as a dry powder and as a frozen solution, but it degrades relatively quickly in liquid media or stock solutions.

Uses

Used in Cell Culture Applications:
L-GLUTAMINE (5-13C) is used as a crucial component in culture media for providing an essential energy source to cells in culture. Its supplementation is necessary for optimal cell performance prior to the use of the media.
Used in Organic Synthesis and Chemical Processes:
L-Glutamine (5-13C, 99%) is utilized as a valuable isotopic labeled intermediate in organic synthesis and other chemical processes, contributing to the development and understanding of various chemical reactions and applications.

Upstream product

• 81202-00-8 (5-13C)-L-Glutamic acid 
• 554428-56-7 (2S,3S,4R)-[3,4-(2)H2;1,2,3,4,5-(13)C5;2-(15)N]glutamic acid 

Relevant articles and documents

Enzymatic synthesis of specifically 2H-labelled L-glutamic acids and 2H-, 15N-, 13C-labelled L-glutamines on a preparative scale

In this paper we report the preparation od specifically 2H-labelled L-glutamic acids and 2H-, 15N-, 13C-labelled L-glutamines on the gram scale.The products obtained have high isotope enrichment (>98percent) and high optical purity.The synthetic schemes allow the specific isotope enrichment of every H, C, and N position or any combination of positions. (2-2H)-L-Glutamic acid was synthesized by enantioselective enzymatic conversion of 2-oxoglutaric acid using glutamate dehydrogenase (GDH, E.C. 1.4.1.3.), alcohol dehydrogenase (ADH, E.C. 1.1.1.1.) and (2H6)ethanol. (3,3-2H2)-L-Glutamic acid was prepared by enzymatic conversion of 2-oxo-3,3-dideuteroglutaric acid which was easily obtained from 2-oxoglutaric acid by an isotope exchange reaction in 2H2O at pH 13.0. (4,4-2H2)-L-Glutamic acid was obtained by chemical exchange in 20percent 2HCl.Four different isotopomers of L-glutamine were synthesized by the enantioselective conversion of isotopically labelled L-glutamic acids using glutamine synthetase (GS, E.C. 6.3.1.2.).The amide group of glutamine was labelled with 15N using 15NH4Cl in the enzymatic reaction.The labelled L-glutamic acids and L-glutamines were characterized by 1H-NMR, 13C-NMR and mass spectroscopy.

STABLE ISOTOPE-LABELED AMINO ACID, METHOD OF INTEGRATING THE SAME INTO TARGET PROTEIN, METHOD OF NMR STRUCTURAL ANALYSIS OF PROTEIN AND PROCESS FOR PRODUCING SITE-SELECTIVE STABLE ISOTOPE-LABELED FUMARIC ACID AND TARTARIC ACID

The present invention provides a stable isotope-labeled amino acid which is at least one of amino acids constituting a protein and which has at least one of the following labeling patterns:(a) hydrogen atoms except at least one hydrogen atom in one or more methylene groups are deuterated,(b) hydrogen atoms in one of prochiral gem-methyl groups are completely deuterated,(c) hydrogen atoms in prochiral methyl groups are partially deuterated, and(d) all hydrogen atoms except one of them in methyl group are deuterated and hydrogen atoms in the aromatic ring are partially deuterated. With the stable isotope-labeled amino acid, the deuteration of protein can be attained without damaging the NMR sensitivity of remaining hydrogen nucleus and, in addition, the rapid, accurate analysis of NMR spectrum of a high-molecular protein which is beyond the limitation in the prior art and the determination of the stereo-structure can be performed at the same time.