161180-11-6 Usage
Description
UMBELLIFERYL ARACHIDONATE, also known as 7-hydroxycoumarinyl Arachidonate, is the arachidonic acid ester of 7-hydroxycoumarin (umbelliferone). It is characterized by its ability to act as a substrate for cPLA2, a phospholipase enzyme. Upon hydrolysis by phospholipase, UMBELLIFERYL ARACHIDONATE releases the fluorescent compound 7-hydroxycoumarin, which can be monitored spectrophotometrically.
Uses
Used in Enzyme Assays:
UMBELLIFERYL ARACHIDONATE is used as a PLA2 fluorogenic substrate for enzyme assays involving phospholipid and Ca2+. Its nonspecific nature makes it suitable for use with purified enzymes, allowing researchers to study the activity and function of phospholipases in various biological processes.
Used in Drug Discovery and High-Throughput Screening (HTS):
UMBELLIFERYL ARACHIDONATE is employed as a valuable tool in drug discovery and high-throughput screening (HTS) applications. Its ability to release a fluorescent compound upon hydrolysis enables the monitoring of enzyme activity in real-time, facilitating the identification of potential drug candidates and the evaluation of their efficacy against specific targets.
Biological Activity
7-hydroxycoumarinyl-arachidonate (7-hca), a fluorogenic substrate for monoacylglycerol lipase (magl), can be used with magl as a novel fluorescence-based assay to screen small molecule inhibitors of magl.magl protein catalyzed the hydrolysis of 7-hca to generate arachidonic acid and the highly fluorescent 7-hydroxyl coumarin (7-hc). 7-hydroxycoumarin (7-hc) can be monitored spectrophotometrically (excitation at 335 nm, emission at 450 nm). release of 7-hc was measured using a fluorometer. magl protein catalyzed the hydrolysis of 7-hca with an apparent km of 9.8 μm and vmax of 1.7 mmol/min/mg of protein. the assay is specific for magl as assay buffer alone or heat-denatured magl protein showed no significant activity against 7-hca [1]. 7-hydroxycoumarinyl arachidonate is the arachidonic acid ester of 7-hydroxycoumarin (umbelliferone) and can be used as a substrate for cpla2. hydrolysis of 7-hydroxycoumarinyl arachidonate by phospholipase resulted in the release of the fluorescent compound [2,3].
references
[1]. wang y, chanda p, jones p g, et al. a fluorescence-based assay for monoacylglycerol lipase compatible with inhibitor screening[j]. assay and drug development technologies, 2008, 6(3): 387-393.[2]. huang z, laliberte f, tremblay n m, et al. a continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase a2[j]. analytical biochemistry, 1994, 222(1): 110-115.[3]. pickard r t, chiou x g, strifler b a, et al. identification of essential residues for the catalytic function of 85-kda cytosolic phospholipase a2 probing the role of histidine, aspartic acid, cysteine, and arginine[j]. journal of biological chemistry, 1996, 271(32): 19225-19231.
Check Digit Verification of cas no
The CAS Registry Mumber 161180-11-6 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,6,1,1,8 and 0 respectively; the second part has 2 digits, 1 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 161180-11:
(8*1)+(7*6)+(6*1)+(5*1)+(4*8)+(3*0)+(2*1)+(1*1)=96
96 % 10 = 6
So 161180-11-6 is a valid CAS Registry Number.
