161401-82-7

Basic information

• Product Name: Z-Vad-fmk, non-methylated
• Synonyms: Carbobenzyloxy-Val-Ala-Asp-alpha-fluoromethylketone;L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-1-(carboxymethyl)-3-fluoro-2-oxopropyl]-;L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-1-(carboxymethyl)-3-fluoro-2-oxopropyl]- (9ci);L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[1-(carboxymethyl)-3-fluoro-2-oxopropyl]-, (S)-;Z-VAD-FMK, Free Acid;Z-VAD-FMK (Caspase Inhibitor VI)
• CAS NO: 161401-82-7
• Molecular Formula: C21H28FN3O7
• Molecular Weight: 453.4613232
• Mol File: 161401-82-7.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 758°Cat760mmHg
• Flash Point: 412.2°C
• Appearance: /
• Density: 1.26g/cm³
• Vapor Pressure: 3.78E-24mmHg at 25°C
• Refractive Index: 1.525
• Storage Temp.: -20C
• PKA: 3.91±0.19(Predicted)
• CAS DataBase Reference: Z-Vad-fmk, non-methylated(CAS DataBase Reference)
• NIST Chemistry Reference: Z-Vad-fmk, non-methylated(161401-82-7)
• EPA Substance Registry System: Z-Vad-fmk, non-methylated(161401-82-7)

Safety Data

• Hazardous Substances Data: 161401-82-7(Hazardous Substances Data)

Usage

Description

Z-Vad-fmk, non-methylated, also known as Z-VAD(OH)-FMK, is an irreversible tripeptide inhibitor of all caspases. It is a valuable tool for research involving recombinant, isolated, and purified caspase enzymes. Unlike the methylated form, Z-VAD(OMe)-FMK, this compound does not require pretreatment with esterases before in vitro use, making it more convenient for various studies.

Uses

Used in Research and Development:
Z-Vad-fmk, non-methylated is used as a research tool for studying the role of caspases in various biological processes. It helps researchers understand the mechanisms of apoptosis and cell death, as well as the potential therapeutic applications of caspase inhibition.
Used in Drug Discovery:
Z-Vad-fmk, non-methylated is used as a lead compound in the development of new drugs targeting caspases. By inhibiting caspase activity, this compound can potentially be used to treat conditions where caspase overactivation is a concern, such as neurodegenerative diseases, autoimmune disorders, and certain types of cancer.
Used in Cell Culture Studies:
Z-Vad-fmk, non-methylated is used as a cell culture reagent to prevent apoptosis in various cell types. This allows researchers to maintain cell viability for longer periods, facilitating the study of cellular processes and the effects of different treatments on cell survival.
Used in Apoptosis Assays:
Z-Vad-fmk, non-methylated is used as a control agent in apoptosis assays to determine the extent of caspase-mediated cell death. By comparing the effects of the inhibitor with untreated samples, researchers can gain insights into the role of caspases in the apoptotic process.
Used in Pharmaceutical Industry:
Z-Vad-fmk, non-methylated is used as a starting point for the development of new pharmaceuticals targeting caspase-mediated diseases. Its ability to inhibit caspase activity makes it a promising candidate for the treatment of conditions where caspase inhibition could be beneficial, such as certain types of cancer and neurodegenerative diseases.

Biochem/physiol Actions

Product does not compete with ATP.

InChI:InChI=1/C21H28FN3O7/c1-12(2)18(25-21(31)32-11-14-7-5-4-6-8-14)20(30)23-13(3)19(29)24-15(9-17(27)28)16(26)10-22/h4-8,12-13,15,18H,9-11H2,1-3H3,(H,23,30)(H,24,29)(H,25,31)(H,27,28)/t13-,15-,18-/m0/s1