169332-61-0

Basic information

• Product Name: AC-DEVD-AMC
• Synonyms: N-ACETYL-ASP-GLU-VAL-ASP-7-AMINO-4-METHYLCOUMARIN AMC;AC-ASP-MET-GLN-ASP-AMC;AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN;AC-ASP-GLU-VAL-ASP-MCA;AC-ASP-GLU-VAL-ASP-7-AMINO-4-METHYLCOUMARIN;AC-ASP-GLU-VAL-ASP-AMC;AC-ASP-GLU-VAL-ASP-AMC AMMONIUM SALT;AC-DEVD-AMC
• CAS NO: 169332-61-0
• Molecular Formula: C30H37N5O13
• Molecular Weight: 675.64
• Product Categories: peptide;Pepetides
• Mol File: 169332-61-0.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 1200.1 °C at 760 mmHg
• Flash Point: 679.6 °C
• Appearance: white/powder
• Density: 1.423 g/cm³
• Storage Temp.: −20°C
• Solubility: DMSO: 10 mM
• PKA: 4.09±0.10(Predicted)
• CAS DataBase Reference: AC-DEVD-AMC(CAS DataBase Reference)
• NIST Chemistry Reference: AC-DEVD-AMC(169332-61-0)
• EPA Substance Registry System: AC-DEVD-AMC(169332-61-0)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 169332-61-0(Hazardous Substances Data)

Usage

Description

AC-DEVD-AMC, also known as N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin, is a synthetic peptide substrate that is widely used in the assessment of caspase-3 activity. It is a fluorogenic substrate, meaning that it emits fluorescence upon cleavage by caspase-3, allowing for the quantification of enzyme activity. AC-DEVD-AMC is a valuable tool in research settings for studying the mechanisms of apoptosis and the effects of various compounds on caspase-3 activity.

Uses

Used in Biomedical Research:
AC-DEVD-AMC is used as a substrate for caspase-3 assay in various cell types, including human colon cancer cell lines and bone marrow mesenchymal stem cells. The reason for its use in these applications is to evaluate the activity of caspase-3, a key enzyme involved in the execution of apoptosis, which is crucial for understanding the mechanisms of cell death and the potential therapeutic effects of compounds on apoptosis regulation.

Biochem/physiol Actions

Fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase.