17695-46-4

Basic information

• Product Name: 4-METHYLUMBELLIFERYL BUTYRATE
• Synonyms: 4-methyl-2-oxo-2H-1-benzopyran-7-yl butyrate;4-METHYLUMBELLIFERYL BUTYRATE, FOR FLUOR ESCENCE;forfluorescence,95+%;mu-bu;4-METHYLUMBELLIFERYL BUTYRATE FOR FLUORESCENCE 95+%;Butyric acid 2-oxo-4-methyl-2H-1-benzopyran-7-yl ester;Butyric acid 4-methyl-2-oxo-2H-1-benzopyran-7-yl ester;METHYLUMBELLIFERYL BUTYRATE, 4-
• CAS NO: 17695-46-4
• Molecular Formula: C₁₄H₁₄O₄
• Molecular Weight: 246.26
• EINECS: 241-694-7
• Product Categories: Substrates
• Mol File: 17695-46-4.mol
• Article Data: 5

Chemical Properties

• Melting Point: 90-92 °C(lit.)
• Boiling Point: 393.4 °C at 760 mmHg
• Flash Point: 199.8 °C
• Appearance: /
• Density: 1.197 g/cm³
• Vapor Pressure: 2.13E-06mmHg at 25°C
• Refractive Index: 1.545
• Storage Temp.: −20°C
• Solubility: DMF: soluble
• BRN: 227973
• CAS DataBase Reference: 4-METHYLUMBELLIFERYL BUTYRATE(CAS DataBase Reference)
• NIST Chemistry Reference: 4-METHYLUMBELLIFERYL BUTYRATE(17695-46-4)
• EPA Substance Registry System: 4-METHYLUMBELLIFERYL BUTYRATE(17695-46-4)

Safety Data

• Hazard Codes: T+
• Statements: 26/27/28
• Safety Statements: 24/25
• WGK Germany: 3
• F: 8-10-21
• Hazardous Substances Data: 17695-46-4(Hazardous Substances Data)

Usage

Description

4-METHYLUMBELLIFERYL BUTYRATE, also known as a member of the coumarins class, is a chemical compound in which the hydroxyl hydrogen of 4-methylumbelliferone is replaced by a butyryl group. It is characterized by its ability to serve as a fluorogenic substrate for esterases and lipases, such as butyrate esterase.

Uses

Used in Enzyme Assays:
4-METHYLUMBELLIFERYL BUTYRATE is used as a fluorogenic substrate for esterases/lipases, such as butyrate esterase, for the purpose of enzyme activity measurement and analysis. This application is particularly useful in research and diagnostic laboratories to study the function and properties of esterases and lipases.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, 4-METHYLUMBELLIFERYL BUTYRATE is used as a key component in the development of drugs targeting esterases and lipases. Its role as a fluorogenic substrate allows for the assessment of drug efficacy and potential interactions with these enzymes, contributing to the advancement of novel therapeutics.
Used in Research and Development:
4-METHYLUMBELLIFERYL BUTYRATE is employed as a research tool in the field of biochemistry and molecular biology. It is utilized to study the mechanisms of action, substrate specificity, and inhibition of esterases and lipases, providing valuable insights into the structure-function relationships of these enzymes and their role in various biological processes.

InChI:InChI=1/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3

Upstream product

• 106-31-0 butanoic acid anhydride 
• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 141-75-3 butyryl chloride 

Downstream Products

• 90104-80-6 8-butyryl-7-hydroxy-4-methyl-coumarin 
• 35031-57-3 1-(2,4-dimethylphenyl)butan-1-one 
• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 107-92-6 butyric acid 
• 623-42-7 butanoic acid methyl ester 
• 105-54-4 butanoic acid ethyl ester 

Relevant articles and documents

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METHOD FOR DETECTION AND/OR ASSAY OF LOVASTATIN ESTERASE WITH USE OF FLUOROGENIC/CHROMOGENIC REAGENT, LOVASTATIN ESTERASE ISOLATED AND/OR PURIFIED BY THIS METHOD, ASSEMBLY FOR DETECTION AND/OR ASSAY AND USE OF FLUOROGENIC/CHROMOGENIC REAGENT FOR DETECTION AND/OR ASSAY OF LOVASTATIN ESTERASE

The invention relates to a method for detection and/or assay of the lovastatin esterase enzymatic activity, with use of the fiuorogenic/chromogenic reagent, comprising use of the fiuorogenic/chromogenic reagent of formula II wherein R represents hydrogen or C1-C6-alkyl, R' represents one or two substituents of the ring A, R" represents one or two or three substituents of the ring B. According to a method, the reagent is added to a material of enzymatic activity and the hydrolysis reaction under enzyme action is carried out, followed by detection and/or assay of the liberated coumarin compound of formula III. Also the invention relates to lovastatin esterase isolated and/or purified using this method, an assembly for detection and/or assay of the lovastatin esterase enzymatic activity, an use of the fiuorogenic/chromogenic reagent of formula II for detection and/or assay of the lovastatin esterase enzymatic activity.

A high-throughput, low-volume enzyme assay on solid support

A high-throughput enzyme assay is described that uses 1 μL or less of enzyme solution for each test Enzyme solutions are deposited by robotic handling in a throughput of over 1000 tests/h on the surface of silica gel plates that have been preimpregnated with fluorogenic substrates. The reaction is quantitated by fluorescence. The method is compatible with water-insoluble substrates (lipases), water-soluble substrates (glycosidases), whole-protein substrates (proteases), and enzyme inhibition measurements. Hydrolytically labile umbelliferyl esters can be used to assay lipases in this format without background hydrolysis. High throughput and reproducibility were tested by fingerprint analysis of lipases and esterases against 37 different fluorogenic ester substrates. A set of eight fluorogenic unbelliferyl esters was selected for optimal activity screening of lipases and esterases on silica gel plates.