17817-20-8

Basic information

• Product Name: FDG
• Synonyms: FLUORESCEIN DI-BETA-D-GALACTOPYRANOSIDE;FLUORESCEIN DI-BETA-D-GALACTOSIDE;fluorescein-digalactoside;FLUORESCEIN DI(BETA-D-GALACTOPYRANOSIDE) , FOR FLUORESCENCE;FLUORESCEIN DI-(B-D-GALACTOPYRANOSIDE);Fluoresceindi--D-Galactopyranside(FDG);fluorescein di(β-d-galactopyranoside);Fluorescein di-β-D-galactopyranoside ,98%
• CAS NO: 17817-20-8
• Molecular Formula: C32H32O15
• Molecular Weight: 656.59
• EINECS: 1312995-182-4
• Product Categories: marker
• Mol File: 17817-20-8.mol
• Article Data: 0

Chemical Properties

• Melting Point: 200-203 °C (dec.)
• Boiling Point: 974.8 °C at 760 mmHg
• Flash Point: 316.5 °C
• Appearance: yellow Powder
• Density: 1.74 g/cm³
• Vapor Pressure: 0mmHg at 25°C
• Refractive Index: 1.764
• Storage Temp.: −20°C
• PKA: 12.44±0.70(Predicted)
• CAS DataBase Reference: FDG(CAS DataBase Reference)
• NIST Chemistry Reference: FDG(17817-20-8)
• EPA Substance Registry System: FDG(17817-20-8)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 8
• Hazardous Substances Data: 17817-20-8(Hazardous Substances Data)

Usage

Description

Fluorescein-digalactoside, also known as FDG, is a synthetic compound that consists of a fluorescein molecule linked to two galactose sugars. It exhibits fluorescent properties upon binding to specific receptors or enzymes, making it a valuable tool in various biological applications.

Uses

Used in Biotechnology Industry:
Fluorescein-digalactoside is used as a detection agent for gene expression via fluorescence-activated cell sorting (FACS). Its fluorescent properties allow for the identification and quantification of specific cell populations expressing target genes.
Used in Molecular Biology Research:
Fluorescein-digalactoside is used as a substrate for detecting glycosidases, enzymes that cleave glycosidic bonds between sugars. It can be utilized in enzyme-linked immunosorbent assays (ELISAs) to study the activity and function of these enzymes.
Used in Genetic Engineering:
Fluorescein-digalactoside is used as a marker for identifying lacZ-positive cells, which contain the lacZ gene responsible for lactose metabolism. This allows researchers to track and visualize the expression of the lacZ gene in living cells using fluorescence microscopy and flow cytometry.
Used in Enzyme Kinetics Studies:
Fluorescein-digalactoside serves as an ultra-sensitive fluorogenic substrate for β-galactosidase, an enzyme involved in lactose metabolism. It is used in live cell and tissue assays (both in vivo and in vitro) to monitor the kinetics of hydrolysis, providing valuable insights into enzyme activity and function.

Biochem/physiol Actions

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

InChI:InChI=1/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1