1866-16-6 Usage
Description
S-Butyrylthiocholine Iodide is a white crystalline powder that serves as a chromogenic substrate for cholinesterases, specifically acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Its chemical properties allow for the colorimetric measurement of enzyme activity at 405 nm, making it a valuable tool in the study and analysis of cholinesterase enzymes.
Uses
Used in Enzyme Activity Assays:
S-Butyrylthiocholine Iodide is used as a chromogenic substrate for the measurement of cholinesterase enzyme activity. The cleavage of this substrate by AChE and BChE can be monitored colorimetrically at 405 nm, providing a quantitative assessment of enzyme activity levels.
Used in Pharmaceutical Research:
In the pharmaceutical industry, S-Butyrylthiocholine Iodide is used as a research tool to study the effects of potential drugs on cholinesterase enzymes. This helps in the development of medications targeting these enzymes, which play a crucial role in various neurological processes.
Used in Environmental Monitoring:
S-Butyrylthiocholine Iodide can be employed in environmental studies to assess the presence and impact of cholinesterase-inhibiting pollutants, such as pesticides, in the ecosystem. By measuring the activity of these enzymes in affected organisms, researchers can gain insights into the extent of contamination and its effects on wildlife.
Used in Educational Settings:
In academic and educational settings, S-Butyrylthiocholine Iodide serves as a practical tool for teaching students about enzyme kinetics, substrate specificity, and the role of cholinesterases in biological systems. This hands-on experience can help students better understand the underlying principles of biochemistry and enzymology.
Purification Methods
Recrystallise S-butyryl thiocholine iodide from propan-1-ol and dry it in vacuo; store it in the dark under N2. The bromide has m 150o (from Me2CO) or m 140-143o (from butan-1-ol). [Gillis Chem Ind (London) 111 1957, Hansen Acta Chem Scand 11 537 1957, Beilstein 4 IV 1586.]
Check Digit Verification of cas no
The CAS Registry Mumber 1866-16-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,8,6 and 6 respectively; the second part has 2 digits, 1 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 1866-16:
(6*1)+(5*8)+(4*6)+(3*6)+(2*1)+(1*6)=96
96 % 10 = 6
So 1866-16-6 is a valid CAS Registry Number.
InChI:InChI=1/C9H20NOS.HI/c1-5-6-9(11)12-8-7-10(2,3)4;/h5-8H2,1-4H3;1H/q+1;/p-1
1866-16-6Relevant articles and documents
Synthetic method for s-butyrylthiocholine iodide
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Paragraph 0039; 0040; 0041, (2018/10/11)
The invention belongs to the technical field of organic synthesis, and provides a synthetic method for s-butyrylthiocholine iodide. The synthetic method comprises the following steps: (1) performing an acylation reaction: performing an esterification reaction on 2-(boc-amino)ethanethiol and butanoyl chloride by using the 2-(boc-amino)ethanethiol as a raw material to obtain s-2-(tert-butoxycarbonylamino)butyryl thiol ethyl ester; (2) performing a deprotection reaction: performing deprotection in the presence of hydrogen chloride by using the s-2-(tert-butoxycarbonylamino)butyryl thiol ethyl ester as a raw material to obtain s-2-aminobutyryl thiol ethyl ester hydrochloride; (3) performing a methylation reaction: performing a reductive amination reaction on the s-2-aminobutyryl thiol ethyl ester hydrochloride and formaldehyde by using the s-2-aminobutyryl thiol ethyl ester hydrochloride as a raw material to obtain s-2-(dimethylamino) butyryl thiol ethyl ester; and 4) performing a quaternization reaction: performing a reaction on the s-2-(dimethylamino) butyryl thiol ethyl ester and iodomethane by using the s-2-(dimethylamino) butyryl thiol ethyl ester as a raw material to obtain the s-butyrylthiocholine iodide. The synthetic method provided by the invention has simple operation, a higher yield and lower costs, and is green, environmentally friendly and suitable for mass preparation.
