191723-65-6

Basic information

• Product Name: H-GLY-GLY-AMC HCL
• Synonyms: GLYCYL-GLYCINE 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE;GLY-GLY-7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE;H-GLY-GLY-AMC HCL;gly-gly-7-amido-4-methylcoumarin
• CAS NO: 191723-65-6
• Molecular Formula: C14H16ClN3O4
• Molecular Weight: 325.75
• Product Categories: Activity;Enzyme Substrates;Fluorogenic
• Mol File: 191723-65-6.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: -15°C
• CAS DataBase Reference: H-GLY-GLY-AMC HCL(CAS DataBase Reference)
• NIST Chemistry Reference: H-GLY-GLY-AMC HCL(191723-65-6)
• EPA Substance Registry System: H-GLY-GLY-AMC HCL(191723-65-6)

Safety Data

• Hazardous Substances Data: 191723-65-6(Hazardous Substances Data)

Usage

Description

H-GLY-GLY-AMC HCL, also known as Gly-Gly-7-amido-4-methylcoumarin, is a synthetic peptide substrate commonly used in the field of biochemistry and molecular biology. It consists of two glycine amino acid residues and a 7-amido-4-methylcoumarin (AMC) group, which serves as a fluorogenic reporter. The molecule is characterized by its ability to be cleaved by proteolytic enzymes, making it a valuable tool for studying enzyme activity and function.

Uses

Used in Biochemical Research:
H-GLY-GLY-AMC HCL is used as a substrate for characterizing a range of bacterial proteolytic enzymes. The reason for its application is that upon cleavage by these enzymes, the AMC group is released, which can be detected by its fluorescence. This allows researchers to monitor enzyme activity, study enzyme specificity, and identify potential enzyme inhibitors.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, H-GLY-GLY-AMC HCL is used as a tool for high-throughput screening of potential drug candidates targeting proteolytic enzymes. The application reason is that the molecule's fluorescence-based detection system enables rapid and sensitive assessment of enzyme inhibition, which is crucial for drug discovery and development.
Used in Diagnostics:
H-GLY-GLY-AMC HCL is also used in the development of diagnostic tests for various diseases, particularly those involving abnormal proteolytic activity. The application reason is that the molecule can serve as a specific and sensitive indicator of enzyme function, which can help in the early detection and monitoring of disease progression.