19916-77-9 Usage
Description
N2-METHYL-2'-DEOXYGUANOSINE is a chemical compound derived from the reaction of formaldehyde with the exocyclic amino group of deoxyguanosine. It plays a significant role in various molecular biology applications due to its unique properties and interactions with other molecules.
Uses
Used in Molecular Biology Research:
N2-METHYL-2'-DEOXYGUANOSINE is used as a template in primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I. This application is crucial for studying DNA replication, repair mechanisms, and the effects of DNA damage on these processes.
Used in Chemical Synthesis:
N2-METHYL-2'-DEOXYGUANOSINE can be employed as a building block or intermediate in the synthesis of more complex molecules, such as modified nucleotides or oligonucleotides, which have potential applications in drug development, diagnostics, and gene therapy.
Used in Analytical Techniques:
N2-METHYL-2'-DEOXYGUANOSINE can be utilized in various analytical techniques to study the interactions between DNA and other molecules, such as proteins or small molecules. Understanding these interactions can provide insights into the mechanisms of DNA recognition, binding, and modification, which are essential for many biological processes.
Used in Pharmaceutical Development:
N2-METHYL-2'-DEOXYGUANOSINE may have potential applications in the development of new drugs targeting DNA-related processes, such as DNA repair, replication, or transcription. By modulating these processes, it may be possible to develop therapies for various diseases, including cancer and genetic disorders.
Used in Environmental Monitoring:
Due to its interaction with formaldehyde, N2-METHYL-2'-DEOXYGUANOSINE can be used as a biomarker for exposure to this harmful compound. Monitoring levels of this compound in biological samples can help assess the risk of formaldehyde exposure and inform public health and safety measures.
Check Digit Verification of cas no
The CAS Registry Mumber 19916-77-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,9,9,1 and 6 respectively; the second part has 2 digits, 7 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 19916-77:
(7*1)+(6*9)+(5*9)+(4*1)+(3*6)+(2*7)+(1*7)=149
149 % 10 = 9
So 19916-77-9 is a valid CAS Registry Number.
19916-77-9Relevant articles and documents
Synthesis of oligodeoxynucleotides containing 6-N-([13C]methyl)adenine and 2-N-([13C]methyl)guanine
Hofmann, Mechtild,Acedo, Montse,Fagan, Patricia,Wemmer, David,Eritja, Ramon,Diaz, Antonio R.
, p. 1825 - 1828 (1997)
Oligonucleotides containing 6-N-([l3C]methyl)adenine and 2-N-([13C]methyl)guanine have been prepared for NMR studies using the deprotection step to introduce the [13C]methylamine group. For this purpose, the use of 2′-deoxy-6-O-(pentafluorophenyl)inosine 1 and 2′-deoxy-2-fluoro-6-O-[2-(4-nitrophenyl)-ethyl]inosine 2 as precursors of the N-methylated nucleosides is described. Preliminary NMR characterization of the 13C-labelled oligonucleotides shows that the 13C chemical shift of the methyl group in N-methylguanine is sensitive to duplex formation, making it a useful local probe.
