201608-14-2 Usage
Description
AC-DEVD-AFC, also known as Acetyl-Asp-Glu-Val-Asp-7-amino-4-trifluoromethylcoumarin, is a synthetic fluorogenic substrate specifically designed for the detection and measurement of caspase-3 and related cysteine proteases' activity. It is a valuable tool in the study of apoptosis, as it allows researchers to monitor the effects of caspase-3 and other caspases on cellular processes.
Uses
Used in Biomedical Research:
AC-DEVD-AFC is used as a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, allowing researchers to study the activity of these enzymes in various cellular processes, particularly apoptosis. The substrate emits a blue to green shift in fluorescence upon cleavage, providing a sensitive and reliable method for detecting caspase activity.
Used in Apoptosis Studies:
AC-DEVD-AFC is used as an essential indicator to monitor the cellular effects of caspase-3, an effector caspase involved in the cleavage of numerous substrates that promote apoptosis. By tracking the activity of caspase-3 using AC-DEVD-AFC, researchers can gain insights into the mechanisms of cell death and develop potential therapeutic strategies for various diseases.
Used in Drug Discovery and Development:
AC-DEVD-AFC is used as a valuable tool in the development of drugs targeting caspase-3 and related proteases. By monitoring the activity of these enzymes in the presence of potential drug candidates, researchers can evaluate the efficacy and selectivity of these compounds in modulating caspase activity and their potential to induce apoptosis.
Used in Diagnostic Applications:
AC-DEVD-AFC can be employed as a diagnostic marker for conditions associated with abnormal apoptosis, such as cancer, neurodegenerative diseases, and autoimmune disorders. By measuring the activity of caspase-3 and related proteases in patient samples, clinicians can assess the extent of apoptosis and monitor the response to therapeutic interventions.
Biological Activity
ac-devd-afc is a fluorogenic substrate for activated caspase-3 (cpp32) with km value of 9.7 μm, and related cysteine proteases [1][2].apoptosis is a process of programmed cell death that occurs in multicellular organisms. caspase are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis, pyroptosis and necroptosis) and inflammation. caspase activation is a major event in apoptosis. caspase-3 cleaves and activates caspases 6 and 7, and is processed and activated by caspases 8, 9, and 10 [1][2][3].ac-devd-afc (n-acetyl-asp-glu-val-asp-7-amido-4-trifluoromethylcoumarin) is a fluorogenic substrate for activated caspase-3 and related caspases. during apoptosis, activated caspase-3 cleaves poly (adp-ribose) polymerase, which it specifically targets at the amino sequence asp-glu-val-asp (devd). sequence is based on parp cleavage at asp216 for caspase-3 [1][2]. caspase activity can be quantified by fluorescent detection of free afc (7-amino-4-trifluoromethylcoumarin) at excitation 400 nm and emission 505 nm [2].
Biochem/physiol Actions
A fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase. The 7-amido-4-trifluoromethylcoumarin derivative has better membrane permeability than the 7-amido-4-methylcoumarin derivative (A1086).
references
[1]. lazebnik ya1, kaufmann sh, desnoyers s, et al. cleavage of poly(adp-ribose) polymerase by a proteinase with properties like ice. nature. 1994 sep 22;371(6495):346-7.[2]. xiang, j.,chao, d.t., and korsmeyer, s.j. bax-induced cell death may not require interleukin 1β-converting enzyme-like proteases. proceedings of the national academy of sciences of the united states of america 93, 14559-14563 (1996).
Check Digit Verification of cas no
The CAS Registry Mumber 201608-14-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,0,1,6,0 and 8 respectively; the second part has 2 digits, 1 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 201608-14:
(8*2)+(7*0)+(6*1)+(5*6)+(4*0)+(3*8)+(2*1)+(1*4)=82
82 % 10 = 2
So 201608-14-2 is a valid CAS Registry Number.
InChI:InChI=1/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1
