206752-36-5 Usage
Description
Benzamidine Hydrochloride Hydrate is a white, fine crystalline powder that serves as a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases. It is widely recognized for its role as a proteolytic inhibitor and apoptosis reagent, making it a valuable compound in various scientific and medical applications.
Uses
Used in Western Blot Analysis:
Benzamidine Hydrochloride Hydrate is used as a protease inhibitor for preventing the degradation of proteins during the western blot analysis process. This ensures the preservation of protein integrity, which is crucial for accurate results in this technique.
Used in Immunoprecipitation:
In immunoprecipitation, Benzamidine Hydrochloride Hydrate acts as a protease inhibitor to prevent the degradation of target proteins. This helps maintain the stability of the protein-antibody complex, allowing for more reliable and accurate immunoprecipitation outcomes.
Used in the Purification of Yeast Dyneins:
Benzamidine Hydrochloride Hydrate is utilized as a proteolytic inhibitor during the purification process of yeast dyneins. This helps protect the dynein motor proteins from degradation, ensuring their structural and functional integrity for further study and analysis.
Used in the Preparation of Brush-Border Membrane Vesicles:
Benzamidine Hydrochloride Hydrate is employed as a protease inhibitor in the preparation of brush-border membrane vesicles. This prevents the non-specific degradation of membrane proteins, which is essential for maintaining the structural and functional properties of the vesicles.
Used in the Processing of Human Synovial Fluid, Blood, and Eye Sleep:
Benzamidine Hydrochloride Hydrate is used as a proteolytic inhibitor in the processing of human synovial fluid, blood, and eye sleep samples. This helps prevent the non-specific degradation of proteins and other biomolecules, ensuring the preservation of their native structure and function for further analysis and study.
Used to Prevent Non-Specific Degradation of Cartilages:
Benzamidine Hydrochloride Hydrate is also used as a protease inhibitor to prevent the non-specific degradation of cartilage tissues. This application is particularly relevant in the study of cartilage biology, joint health, and the development of treatments for conditions such as osteoarthritis.
Biological Activity
Peptidase inhibitor.Benzamidine HCl is a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases.A concentration of approximately 1 mM is used for general protease inhibition. To inhibit proteases from yeast, a range of 0.5 to 4.0 mM is used and it is for the most part interchangeable with pepstatin A.In addition to being a strong competitive inhibitor of trypsin, benzamidine HCl has been also shown to be a strong competitive inhibitor of thrombin and plasmin. It was also found to be as effective as aprotinin in the prevention of glucagon degradation in human plasma.
Biochem/physiol Actions
Peptidase inhibitor.
Purification Methods
Recrystallise it from dilute HCl (crystals contain xH2O) or EtOH/few drops HCl; dry it in a vacuum. It is a proteolytic inhibitor [Jeffcoate & White J Clin Endocrinol Metab 38 155 1974, Beilstein 9 IV 898.]
Preparation and handling
Benzamidine HCl is soluble in water and alcohol. Solubility testing in water at a concentration of 50 mg/ml yields a clear solution with heating. Benzamidine HCl is sensitive to oxidation. It is recommended to prepare solutions fresh each time in degassed water prior to use. However, frozen aliquots stored under inert gas, to exclude air, may be stable for a short time. Insufficient information is available to assess the shelf-life of a frozen solution.
Precautions
Keep container tightly sealed. Store in cool, dry conditions in well sealed containers. Incompatible with oxidizing agents. It is hygroscopic in nature.
Check Digit Verification of cas no
The CAS Registry Mumber 206752-36-5 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,0,6,7,5 and 2 respectively; the second part has 2 digits, 3 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 206752-36:
(8*2)+(7*0)+(6*6)+(5*7)+(4*5)+(3*2)+(2*3)+(1*6)=125
125 % 10 = 5
So 206752-36-5 is a valid CAS Registry Number.
InChI:InChI=1/C7H8N2/c8-7(9)6-4-2-1-3-5-6/h1-5H,(H3,8,9)/p+1
