21653-40-7

Basic information

• Product Name: N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE
• Synonyms: Na-Benzoyl-1-arginine-4-nitroanilidehydrochloride;(S)-5-(amidinoamino)-2-(benzoylamino)-N-(4-nitrophenyl)valeramide monohydrochloride;NA-BENZOYL-L-ARGININE P-NITROANILIDEHYDR OCHLORIDE;N-alpha-benzoyl-L-argininep-nitroanilide;Benzamide, N-(1S)-4-(aminoiminomethyl)amino-1-(4-nitrophenyl)aminocarbonylbutyl-, monohydrochloride;nα-benzoyl-l-arginine 4-nitroanilide hydrochloride;NA-BENZOYL-L-ARGININEP-NITROANILILDEHYDROCHLORIDE;NALPHA-Benzoyl-L-arginine-4-nitroanilide
• CAS NO: 21653-40-7
• Molecular Formula: C19H23ClN6O4
• Molecular Weight: 434.88
• EINECS: 244-505-6
• Mol File: 21653-40-7.mol
• Article Data: 0

Chemical Properties

• Melting Point: 223-226 °C
• Boiling Point: 755.4 °C at 760 mmHg
• Flash Point: 410.7 °C
• Appearance: White to Yellow/Powder
• Density: g/cm³
• Vapor Pressure: 3.65E-23mmHg at 25°C
• Storage Temp.: −20°C
• Solubility: Soluble in DMSO at 50mg/ml
• BRN: 4081878
• CAS DataBase Reference: N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE(CAS DataBase Reference)
• NIST Chemistry Reference: N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE(21653-40-7)
• EPA Substance Registry System: N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE(21653-40-7)

Safety Data

• Safety Statements: 24/25
• WGK Germany: 3
• F: 3-8-10
• Hazardous Substances Data: 21653-40-7(Hazardous Substances Data)

Usage

Description

N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE, also known as L-BAPNA, is a colorless, chromogenic substrate specifically designed for the detection and measurement of proteolytic enzyme activity. It is a light yellow powder that serves as a valuable tool in various research and diagnostic applications due to its ability to change color upon enzymatic cleavage.

Uses

Used in Enzyme Activity Assays:
N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE is used as a chromogenic substrate for the assessment of trypsin, papain, and other proteolytic enzyme activities. The substrate's color change upon enzymatic cleavage allows for the quantification of enzyme activity, making it a crucial component in research and diagnostic laboratories.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE is used as a reagent for the development and testing of new drugs targeting proteolytic enzymes. Its ability to interact with these enzymes provides a means to evaluate the efficacy and specificity of potential therapeutic agents.
Used in Research and Development:
N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE is used as a research tool for the study of enzyme kinetics, mechanism of action, and the development of novel inhibitors or activators for proteolytic enzymes. This substrate is particularly useful in the fields of biochemistry, molecular biology, and pharmacology.
Used in Diagnostic Applications:
In the diagnostic field, N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE is employed as a substrate for the detection and quantification of specific proteolytic enzymes in biological samples. This aids in the diagnosis of various diseases and conditions associated with abnormal enzyme activity.
Used in Quality Control:
N-ALPHA-BENZOYL-L-ARGININE P-NITROANILIDE HYDROCHLORIDE is utilized in the quality control of enzyme preparations, ensuring that the enzymes maintain their activity and specificity throughout the manufacturing process. This is essential for the production of reliable and effective therapeutic and diagnostic products.

InChI:InChI=1/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H

Downstream Products

• 100-01-6 4-nitro-aniline