21653-41-8 Usage
Description
BZ-D-ARG-PNA HCL, also known as Benzoyl-D-arginine 4-Nitroanilide Hydrochloride, is a synthetic chromogenic substrate commonly used in the assessment of the activity of specific proteolytic enzymes, such as trypsin or papain. Its structure allows for a colorimetric change upon enzymatic cleavage, making it a valuable tool in various research and diagnostic applications.
Uses
Used in Research and Diagnostic Applications:
BZ-D-ARG-PNA HCL is used as a chromogenic substrate for the detection and quantification of trypsin or papain activity. The substrate's cleavage by these enzymes releases a colored product, 4-nitroaniline, which can be measured spectrophotometrically. This property makes it an essential component in assays designed to study enzyme kinetics, monitor enzyme activity, and evaluate the effectiveness of protease inhibitors.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, BZ-D-ARG-PNA HCL serves as a valuable tool for the development and testing of new drugs targeting proteolytic enzymes. By incorporating this substrate into assays, researchers can assess the potency and selectivity of potential inhibitors, aiding in the optimization of drug candidates and facilitating the discovery of novel therapeutic agents.
Used in Quality Control and Validation:
BZ-D-ARG-PNA HCL is also utilized in the quality control and validation processes of proteolytic enzyme preparations. By measuring the enzyme's activity using this substrate, manufacturers can ensure the consistency, purity, and potency of their enzyme products, ultimately contributing to the safety and efficacy of these enzymes in various applications.
Used in Educational Settings:
In educational settings, BZ-D-ARG-PNA HCL can be employed as a teaching aid to demonstrate the principles of enzyme kinetics and the mechanisms of proteolytic enzymes. By conducting experiments with this substrate, students can gain hands-on experience in understanding the relationship between enzyme activity and substrate concentration, as well as the factors that influence enzyme function.
Check Digit Verification of cas no
The CAS Registry Mumber 21653-41-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,1,6,5 and 3 respectively; the second part has 2 digits, 4 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 21653-41:
(7*2)+(6*1)+(5*6)+(4*5)+(3*3)+(2*4)+(1*1)=88
88 % 10 = 8
So 21653-41-8 is a valid CAS Registry Number.
