23491-45-4 Usage
Description
Hoechst 33258, also known as bisBenzimide H 33258, is a membrane-permeable, adenine-thymine-specific fluorescent stain. It is a yellow to yellow-green powder that is primarily used for staining DNA, chromosomes, and nuclei. The dye binds to the minor groove of DNA at AT-rich sequences, making it a valuable tool in various applications.
Uses
Used in Biotechnology and Research:
Hoechst 33258 is used as a fluorescent stain for DNA, chromosomes, and nuclei for fluorescence microscopy or flow cytometry. It is particularly useful for determining the DNA content of viable cells without the need for detergent treatment or fixation. The dye has an excitation maximum at 346 nm and an emission maximum at 460 nm.
Used in Tissue Culture:
Hoechst 33258 is used as a detection agent for mycoplasma contamination in tissue culture. Its ability to bind to DNA allows for the identification and monitoring of mycoplasma infections, which can compromise the integrity of cell cultures.
Used in Gene Expression Control:
Hoechst 33258 and its related compound H 33342 have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control the expression of transfected gene constructs. This application aids in the regulation of gene expression in various research and therapeutic contexts.
Used in Calcium Efflux Studies:
Hoechst 33258 increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. This property makes it a valuable tool in studying the mechanisms of calcium signaling and its role in cellular processes.
Biochem/physiol Actions
bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.
Check Digit Verification of cas no
The CAS Registry Mumber 23491-45-4 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,3,4,9 and 1 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 23491-45:
(7*2)+(6*3)+(5*4)+(4*9)+(3*1)+(2*4)+(1*5)=104
104 % 10 = 4
So 23491-45-4 is a valid CAS Registry Number.
InChI:InChI=1/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
